Jittmittraphap Akanitt, Thammapalo Suwich, Ratanasetyuth Nitipon, Wongba Narong, Mammen Mammen P, Jampangern Wipawee
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2006 Nov;37(6):1117-24.
RNA amplification by nucleic acid sequence-based amplification (NASBA) was used to detect serotype specific dengue viruses in artificially-infected female Aedes mosquitoes, in comparison with RT-PCR technique. NASBA could detect dengue virus serotype 2 and 4 below 0.1 PFU, which was more sensitive than RT-PCR, but this technique was as sensitive as RT-PCR when detecting dengue virus serotype 1 and 3. Dengue viruses could be detected at the thorax of mosquitoes at 0, 7, and 14 days after inoculation with dengue virus serotype 2. This method should be useful for virological surveillance of dengue infected Aedes mosquitoes, as an early warning system to predict outbreaks of dengue viruses.
与逆转录聚合酶链反应（RT-PCR）技术相比，基于核酸序列扩增（NASBA）的RNA扩增技术用于检测人工感染的雌性伊蚊体内血清型特异性登革病毒。NASBA能够检测到低于0.1 PFU的登革病毒血清型2和4，比RT-PCR更灵敏，但在检测登革病毒血清型1和3时，该技术与RT-PCR的灵敏度相当。在接种登革病毒血清型2后的第0、7和14天，可在蚊子的胸部检测到登革病毒。作为预测登革病毒爆发的早期预警系统，该方法应有助于对登革热感染的伊蚊进行病毒学监测。
