Marbawati Dewi, Umniyati Sitti Rahmah
Vector Borne Disease Control Research and Development Office in Banjarnegara, Selamanik Street Banjarnegara 53415, Indonesia.
Departemen of Parasitology, Faculty of Medicine, Universitas Gadjah Mada, Sekip Utara, Yogyakarta,55281, Indonesia.
Procedia Environ Sci. 2015;23:215-221. doi: 10.1016/j.proenv.2015.01.033. Epub 2015 Jan 31.
The research aims was to determine the cytotoxic effects and the highest safe concentration of curcumin and PGV-0 on vero cells. This research also compare the effect of curcumin and PGV-0 against vero cells infected by Dengue virus (in vitro) in one and three days incubation period. The highest safe concentrations against vero cells from curcumin was 6.25 ppm and 1.5625 ppm for PGV-0. Immunocytochemistry test of curcumin and PGV-0 in both incubation period (one and three days) shows no significant difference (significant value 0.925), but RT-PCR result indicates that PGV - 0 have potential antiviral better than curcumin.
该研究旨在确定姜黄素和PGV-0对非洲绿猴肾细胞（Vero细胞）的细胞毒性作用及最高安全浓度。本研究还比较了姜黄素和PGV-0在体外培养1天和3天的情况下对登革病毒感染的Vero细胞的作用。姜黄素对Vero细胞的最高安全浓度为6.25 ppm，PGV-0为1.5625 ppm。在两个培养期（1天和3天）对姜黄素和PGV-0进行的免疫细胞化学测试显示无显著差异（显著性值为0.925），但逆转录聚合酶链反应（RT-PCR）结果表明，PGV-0的潜在抗病毒能力优于姜黄素。
