Prakash Anil, Walton C, Bhattacharyya D R, Mohapatra P K, Mahanta J
Regional Medical Research Center, NE (Indian Council of Medical Research), Dibrugarh, Assam, India.
Southeast Asian J Trop Med Public Health. 2006 Nov;37(6):1134-8.
Anopheles (Cellia) philippinensis Ludlow and Anopheles (Cellia) nivipes (Theobald) are two closely related, morphologically very similar, mosquito species in the Annularis group, which play a supportive role in malaria transmission in north-east India. We amplified and performed sequence analysis for the internal transcribed spacer 2 (ITS2) locus of ribosomal DNA (rDNA) gene of morphologically confirmed specimens of these two species from the states of Assam and Nagaland. An. philippinensis and An. nivipes shared 85.2% sequence similarity and no intra-species variation was found in the nucleotide sequences of the two species. Overall, sequence data of the ITS2 marker revealed that both these species from north-east India differed by as much as have been reported from specimens of eastern Thailand.
菲律宾按蚊（塞利亚按蚊）卢德洛亚种和尼氏按蚊（塞利亚按蚊）（西奥博尔德）是环纹按蚊组中两个亲缘关系密切、形态非常相似的蚊种，在印度东北部的疟疾传播中起辅助作用。我们对来自阿萨姆邦和那加兰邦的这两个物种形态学确认标本的核糖体DNA（rDNA）基因的内部转录间隔区2（ITS2）位点进行了扩增和序列分析。菲律宾按蚊和尼氏按蚊的序列相似性为85.2%，且在这两个物种的核苷酸序列中未发现种内变异。总体而言，ITS2标记的序列数据显示，印度东北部的这两个物种之间的差异与泰国东部标本所报道的差异一样大。
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