Regional Medical Research Centre, NE Region, Indian Council of Medical Research, Dibrugarh, Assam, India.
Acta Trop. 2012 Oct;124(1):62-70. doi: 10.1016/j.actatropica.2012.06.011. Epub 2012 Jul 4.
Anopheles (Cellia) maculatus is considered a group of at least nine formally named species. Faced with the difficulty of correct morphological identification due to overlapping characters, several member species of the An. maculatus group are known to play important role in malaria transmission in the Oriental region. Current assemblage, distribution and vectorial importance of the member species within the Maculatus group is far from clear in the north-eastern region of India. Our study encompassing 410 individuals, collected from 67 geo-referenced spots across the eight north-east Indian states, identified the presence of 6 member species of the Maculatus group using the molecular tools. Anopheles dravidicus and Anopheles rampae were documented for the first time in this part of India with latter forming the new country record. While Anopheles pseudowillmori (59.5%) and An. maculatus (32%) were widely available species in most of the north-eastern states, restricted distribution of Anopheles willmori to Nagaland and that of Anopheles sawadwongporni and An. rampae to Mizoram state was noted. None of the species was found positive for human malaria parasite. While no intraspecific differences existed in the sequences of second internal transcribed spacer (ITS2) region of ribosomal DNA (r-DNA) of the member species of the Maculatus group within north-east India, few differences were detected in the sequences of An. dravidicus, An. maculatus and An. pseudowillmori from north-east India with species from the neighbouring countries.
斑蚊（Cellia）maculatus 被认为是至少九个正式命名的物种的群体。由于重叠的特征，正确的形态识别存在困难，已知斑蚊 maculatus 组的几个成员物种在东方地区的疟疾传播中起着重要作用。在印度东北部地区，斑蚊组内成员物种的当前组合、分布和媒介重要性还远不清楚。我们的研究包括从印度东北部 8 个邦的 67 个地理参考点采集的 410 个人，使用分子工具鉴定出了 6 种斑蚊组的成员物种。首次在印度这一地区发现了疟蚊（Dravidicus）和疟蚊（Rampae），后者形成了新的国家记录。虽然在印度东北部的大多数邦都发现了疟蚊（Pseudowillmori）（59.5%）和疟蚊（Maculatus）（32%）这两种广泛存在的物种，但在 Nagaland 发现了疟蚊（Willmori）的分布受限，而在 Mizoram 邦发现了疟蚊（Sawadwongporni）和疟蚊（Rampae）的分布受限。没有发现任何一种蚊子对人类疟原虫呈阳性。虽然在印度东北部斑蚊组内成员物种的核糖体 DNA（r-DNA）的第二内部转录间隔区（ITS2）序列中没有种内差异，但在来自印度东北部的疟蚊（Dravidicus）、疟蚊（Maculatus）和疟蚊（Pseudowillmori）的序列中检测到了一些差异，与来自邻国的物种存在差异。
