Regional Medical Research Centre, NE (Indian Council of Medical Research), Dibrugarh, Assam, India.
Acta Trop. 2012 Jun;122(3):247-54. doi: 10.1016/j.actatropica.2012.02.065. Epub 2012 Feb 25.
Anopheles philippinensis-nivipes species of mosquitoes are considered secondary malaria vectors in the north-east India. However, difficulty in separating An. philippinensis and An. nivipes accurately on the basis of morphological identification, due to the overlapping female wing characters, has led to the paucity of information on their spatial distribution and vectorial importance, essential for understanding malaria disease epidemiology in areas under their influence. In view of this a study was undertaken to generate authentic information on distribution of An. philippinensis and An. nivipes in the north-east India using molecular tools. Between the two, An. nivipes was recorded as the dominant species in Assam, and Nagaland states whereas An. philippinensis was found predominant in the state of Mizoram and Arunachal Pradesh. Species differentiation based on female wing characters proved wrong in about 74% specimens of An. philippinensis but found accurate in case of An. nivipes. No intra-specific variation in second internal transcribed spacer (ITS2) region of ribosomal DNA(r-DNA) in either An. nivipes or An. philippinensis from different geographical areas of north-east India was noted.
在印度东北部，按蚊菲律宾-尼维斯亚种被认为是次要的疟疾传播媒介。然而，由于雌性翅膀特征的重叠，在形态学鉴定的基础上准确区分按蚊菲律宾-尼维斯亚种和按蚊尼维斯亚种存在困难，这导致了对其空间分布和媒介重要性的信息匮乏，而这些信息对于理解受其影响地区的疟疾疾病流行病学至关重要。有鉴于此，一项使用分子工具研究在印度东北部生成按蚊菲律宾-尼维斯亚种和按蚊尼维斯亚种分布的真实信息的研究已经展开。在这两种蚊子中，按蚊尼维斯亚种在阿萨姆邦和那加兰邦被记录为优势物种，而按蚊菲律宾-尼维斯亚种在米佐拉姆邦和阿鲁纳恰尔邦则更为普遍。基于雌性翅膀特征的物种分化在约 74%的按蚊菲律宾-尼维斯亚种标本中被证明是错误的，但在按蚊尼维斯亚种中则是准确的。在印度东北部不同地理区域的按蚊尼维斯亚种或按蚊菲律宾-尼维斯亚种的核糖体 DNA(r-DNA)的第二内部转录间隔区 (ITS2) 区域没有观察到种内变异。
