Barbosa C R, Albuquerque E M V, Faria E C, Oliveira H C F, Castilho L N
Departamento de Patologia Clínica e Núcleo de Medicina e Cirurgia Experimental, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, 13084-971 Campinas, SP, Brazil.
Braz J Med Biol Res. 2007 Mar;40(3):323-31. doi: 10.1590/s0100-879x2007000300007.
The metabolic effects of carbohydrate supplementation in mice have not been extensively studied. In rats, glucose- and fructose-rich diets induce hypertriacylglycerolemia. In the present study, we compared the metabolic responses to two monosaccharide supplementations in two murine models. Adult male Wistar rats (N = 80) and C57BL/6 mice (N = 60), after 3 weeks on a standardized diet, were submitted to dietary supplementation by gavage with glucose (G) or fructose (F) solutions (500 g/L), 8 g/kg body weight for 21 days. Glycemia was significantly higher in rats after fructose treatment (F: 7.9 vs 9.3 mM) and in mice (G: 6.5 vs 10 and F: 6.6 vs 8.9 mM) after both carbohydrate treatments. Triacylglycerolemia increased significantly 1.5 times in rats after G or F supplementation. Total cholesterol did not change with G treatment in rats, but did decrease after F supplementation (1.5 vs 1.4 mM, P < 0.05). Both supplementations in rats induced insulin resistance, as suggested by the higher Homeostasis Model Assessment Index. In contrast, mice showed significant decreases in triacylglycerol (G: 1.8 vs 1.4 and F: 1.9 vs 1.4 mM, P < 0.01) and total cholesterol levels (G and F: 2.7 vs 2.5 mM, P < 0.05) after both monosaccharide supplementations. Wistar rats and C57BL/6 mice, although belonging to the same family (Muridae), presented opposite responses to glucose and fructose supplementation regarding serum triacylglycerol, free fatty acids, and insulin levels after monosaccharide treatment. Thus, while Wistar rats developed features of plurimetabolic syndrome, C57BL/6 mice presented changes in serum biochemical profile considered to be healthier for the cardiovascular system.
碳水化合物补充对小鼠代谢的影响尚未得到广泛研究。在大鼠中，富含葡萄糖和果糖的饮食会诱发高三酰甘油血症。在本研究中，我们比较了两种小鼠模型对两种单糖补充剂的代谢反应。成年雄性Wistar大鼠（N = 80）和C57BL/6小鼠（N = 60）在标准化饮食3周后，通过灌胃给予葡萄糖（G）或果糖（F）溶液（500 g/L）进行饮食补充，剂量为8 g/kg体重，持续21天。果糖处理后大鼠的血糖显著升高（F：7.9对9.3 mM），两种碳水化合物处理后小鼠的血糖也显著升高（G：6.5对10，F：6.6对8.9 mM）。补充G或F后，大鼠的三酰甘油血症显著增加1.5倍。大鼠经G处理后总胆固醇未发生变化，但经F补充后总胆固醇降低（1.5对1.4 mM，P < 0.05）。如稳态模型评估指数升高所示，两种补充剂在大鼠中均诱导了胰岛素抵抗。相比之下，两种单糖补充后，小鼠的三酰甘油（G：1.8对1.4，F：1.9对1.4 mM，P < 0.01）和总胆固醇水平显著降低（G和F：2.7对2.5 mM，P < 0.05）。Wistar大鼠和C57BL/6小鼠虽然属于同一家族（鼠科），但在单糖处理后，它们对葡萄糖和果糖补充在血清三酰甘油、游离脂肪酸和胰岛素水平方面呈现相反的反应。因此，Wistar大鼠出现了多代谢综合征的特征，而C57BL/6小鼠的血清生化指标变化被认为对心血管系统更健康。
