Venturi Vanessa, Kedzierska Katherine, Turner Stephen J, Doherty Peter C, Davenport Miles P
Department of Haematology, Prince of Wales Hospital and, Centre for Vascular Research, University of New South Wales, Kensington NSW 2052, Australia.
J Immunol Methods. 2007 Apr 10;321(1-2):182-95. doi: 10.1016/j.jim.2007.01.019. Epub 2007 Feb 21.
Analysis of T cell receptor (TCR) data has become a crucial element in many studies aimed at better understanding the evolution of the T cell repertoire and the role of TCR diversity in immune responses. In this paper we focus on comparing the diversity between samples of the TCR repertoire. We discuss some of the limitations and potential problems inherent in some of the more popular approaches to comparing samples of the TCR repertoire and we suggest alternate methods that both avoid these problems and enrich the analysis of TCR data. Examples from published studies of the CD8(+) T cell responses to the influenza A virus D(b)NP(366) and D(b)PA(224) epitopes in mice are used to demonstrate the implementation of these methods. One example involves a comparison between the central and effector memory T cell subsets, defined on the basis of CD62L expression, and the other examines changes in the TCR repertoire over time.
T细胞受体（TCR）数据分析已成为许多旨在更好地理解T细胞库进化以及TCR多样性在免疫反应中作用的研究的关键要素。在本文中，我们专注于比较TCR库样本之间的多样性。我们讨论了一些比较TCR库样本的更流行方法中固有的局限性和潜在问题，并提出了既能避免这些问题又能丰富TCR数据分析的替代方法。已发表的关于小鼠对甲型流感病毒D(b)NP(366)和D(b)PA(224)表位的CD8(+) T细胞反应研究中的例子用于证明这些方法的实施。一个例子涉及基于CD62L表达定义的中枢记忆和效应记忆T细胞亚群之间的比较，另一个例子则研究了TCR库随时间的变化。
