Guo N-N, Li B-M
Laboratory of Higher Brain Functions, Institute of Neurobiology, Institutes of Brain Science, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China.
Neuroscience. 2007 Apr 25;146(1):298-305. doi: 10.1016/j.neuroscience.2007.01.013. Epub 2007 Mar 2.
Beta-adrenoceptors (ARs) in the hippocampus play an important role in regulating synaptic plasticity and memory consolidation. However, little is known about the distributions of beta-ARs in the hippocampus, especially in the cornu ammonis (CA)1 and CA3 regions of Sprague-Dawley rats. Here, we report that beta1- and beta2-ARs in the CA1 and CA3 regions have differential subcellular distributions. Using double immunofluorescence labeling and confocal laser scanning microscopy, we found that almost all of the neuronal nuclei positive cells express beta1- and beta2-ARs, while few glial fibrillary acidic protein positive cells express them. Interestingly, beta1-ARs are predominantly distributed in the cell membrane and cytoplasm, whereas beta2-ARs are predominantly distributed not only in the membrane and cytoplasm, but also in the nucleus. The differential subcellular distribution of beta1- and beta2-ARs may have functional significance.
海马体中的β-肾上腺素能受体（ARs）在调节突触可塑性和记忆巩固方面发挥着重要作用。然而，关于β-ARs在海马体中的分布，尤其是在斯普拉格-道利大鼠的海马角（CA）1和CA3区域的分布，人们了解甚少。在此，我们报告CA1和CA3区域中的β1-和β2-ARs具有不同的亚细胞分布。通过双重免疫荧光标记和共聚焦激光扫描显微镜，我们发现几乎所有神经元核阳性细胞都表达β1-和β2-ARs，而很少有胶质纤维酸性蛋白阳性细胞表达它们。有趣的是，β1-ARs主要分布在细胞膜和细胞质中，而β2-ARs不仅主要分布在细胞膜和细胞质中，还分布在细胞核中。β1-和β2-ARs不同的亚细胞分布可能具有功能意义。
