Zheng Jian, Luo Fei, Guo Nan-nan, Cheng Zong-yue, Li Bao-ming
Center for Neuropsychiatric Diseases, Institute of Life Science, Nanchang University, Nanchang 330031, China.
Center for Neuropsychiatric Diseases, Institute of Life Science, Nanchang University, Nanchang 330031, China.
Brain Res. 2015 Nov 19;1627:109-18. doi: 10.1016/j.brainres.2015.08.035. Epub 2015 Sep 4.
The existence of β-adrenoceptors (ARs) in the hippocampus and the importance of β-ARs in regulating synaptic plasticity and learning/memory function are well documented. As known, β-ARs in area cornu ammonis 1 (CA1) are involved in regulating memory consolidation. However, little is known about the functional roles of the β-ARs subtypes, β1- and β2-ARs, in the hippocampal cornu ammonis 3 (CA3) region. To address this question, we firstly locally infused the β1- or β2-ARs antagonist into the CA3 region and observed that blockage of either β1-AR or β2-AR impaired long-term contextual fear memory and water-maze spatial memory. We also found that, following the contextual fear conditioning, the expression of β1-AR in the CA3 region significantly increased, whereas β2-AR was unchanged. Then intra-CA3 infusion of recombinant lentiviral RNAi vectors for β1 or β2-ARs also produced deficit in contextual memory consolidation. Taken together, the results suggested that the β1- and β2-ARs in the CA3 region were involved in hippocampus dependent memory consolidation.
海马体中β-肾上腺素能受体（ARs）的存在以及β-ARs在调节突触可塑性和学习/记忆功能方面的重要性已有充分记录。众所周知，海马角回1（CA1）区的β-ARs参与调节记忆巩固。然而，关于β-ARs亚型β1-和β2-ARs在海马角回3（CA3）区的功能作用知之甚少。为了解决这个问题，我们首先将β1-或β2-ARs拮抗剂局部注入CA3区，观察到阻断β1-AR或β2-AR均会损害长期情境恐惧记忆和水迷宫空间记忆。我们还发现，在情境恐惧条件反射后，CA3区β1-AR的表达显著增加，而β2-AR则无变化。然后，向CA3区内注入针对β1或β2-ARs的重组慢病毒RNAi载体也会导致情境记忆巩固出现缺陷。综上所述，结果表明CA3区的β1-和β2-ARs参与了海马体依赖的记忆巩固。
