Splinter T A, Helder A W, Lucas C J, Feltkamp-Vroom T M
Br J Exp Pathol. 1975 Apr;56(2):124-32.
Human lymphoid cell lines were studied as an experimental model for the spontaneous or induced occurrence of tuburloreticular structures (TRS). It was possible to induce TRS after culturing the EB-3 cell line with 20 mug/ml bromodeoxyuridine (BrUdR) during 96 h. Starvation, culturing at lower temperature (32 degrees) or inhibition of DNA synthesis did not give rise to the production of TRS. The response to BrUdR could be blocked with 60 mug/ml thymidine but not with 60 mug/ml deoxycytidine. The addition of 5 mug/ml cytarabine or the removal of BrUdR at different times resulted in inhibition of TRS induction, indicating that BrUdR had to be incorporated into DNA during at least 48 h. After incorporation, neither the presence of BrUdR nor DNA synthesis was necessary for the production of TRS. These experiments and the finding that in the cell line IHTC-33, which does not produce Epstein-Barr virus associated antigens, TRS were spontaneously present, exclude a correlation between TRS and these antigens. However, the induction of TRS by BrUdR may be related to the activation of another (latent) virus.
人类淋巴细胞系作为自发或诱导产生管状网状结构（TRS）的实验模型进行了研究。在用20微克/毫升溴脱氧尿苷（BrUdR）培养EB - 3细胞系96小时后，有可能诱导出TRS。饥饿、在较低温度（32摄氏度）下培养或抑制DNA合成均未导致TRS的产生。对BrUdR的反应可用60微克/毫升胸苷阻断，但不能用60微克/毫升脱氧胞苷阻断。添加5微克/毫升阿糖胞苷或在不同时间去除BrUdR会导致TRS诱导受到抑制，这表明BrUdR必须至少在48小时内掺入DNA中。掺入后，TRS的产生既不需要BrUdR的存在，也不需要DNA合成。这些实验以及在不产生爱泼斯坦 - 巴尔病毒相关抗原的IHTC - 33细胞系中自发存在TRS这一发现，排除了TRS与这些抗原之间的相关性。然而，BrUdR诱导TRS可能与另一种（潜伏）病毒的激活有关。
