Roberson D F, Weisleder P, Bohrer P S, Rubel E W
Department of Otolaryngology, Head and Neck Surgery, University of Washington, Seattle 98195.
Hear Res. 1992 Jan;57(2):166-74. doi: 10.1016/0378-5955(92)90149-h.
Recent studies have shown that the vestibular and auditory systems of some species of birds have the capacity to generate sensory hair cells postnatally. We used a traditional technique, 3H-thymidine autoradiography, and a newer method, bromodeoxyuridine immunocytochemistry, to determine whether ongoing proliferation of hair cells occurs in the intact chick vestibular epithelium. A ten-day course of 3H-thymidine, bromodeoxyuridine, or both was administered to twelve-day-old chicks. Both autoradiographic and immunocytochemical labeling demonstrated ongoing production of supporting cells and Type II hair cells in all chick vestibular organs. No evidence for production of Type I hair cells was seen in this investigation. New sensory cells were distributed throughout the epithelium; there was no peripheral growth zone analogous to that found in other vertebrates. Labeled Type II hair cells were frequently seen immediately above labeled supporting cells. This observation suggests that supporting cells are precursors for new hair cells. The ongoing, postnatal regeneration of vestibular epithelial cells also suggests that this epithelium may retain the potential for repair after trauma or ototoxic damage.
最近的研究表明，某些鸟类的前庭和听觉系统在出生后具有产生感觉毛细胞的能力。我们使用传统技术（3H-胸腺嘧啶核苷放射自显影术）和一种较新的方法（溴脱氧尿苷免疫细胞化学）来确定完整雏鸡前庭上皮中是否存在毛细胞的持续增殖。给12日龄雏鸡施用为期十天的3H-胸腺嘧啶核苷、溴脱氧尿苷或两者。放射自显影和免疫细胞化学标记均表明，所有雏鸡前庭器官中均存在支持细胞和II型毛细胞的持续产生。在本研究中未发现I型毛细胞产生的证据。新的感觉细胞分布于整个上皮；没有类似于其他脊椎动物中发现的外周生长区。标记的II型毛细胞经常紧邻标记的支持细胞上方出现。这一观察结果表明，支持细胞是新毛细胞的前体。前庭上皮细胞在出生后的持续再生也表明，该上皮在受到创伤或耳毒性损伤后可能保留修复潜力。
