Weisleder P, Tsue T T, Rubel E W
Virginia Merrill Bloedel Hearing Research Center, Seattle, Washington, USA.
Hear Res. 1995 Jan;82(1):125-33. doi: 10.1016/0378-5955(94)00169-q.
Previous investigations have demonstrated that the sensory epithelium of the avian vestibular system possesses the capacity to replace hair cells both on an ongoing basis and following severe damage. Supporting cells, within the sensory epithelium, are believed to be the progenitors of the regenerated hair cells. In the present study we describe the series of events leading to the formation of a regenerated vestibular hair cell in post-hatched birds. Young chickens received injections of streptomycin sulfate in order to damage the sensory epithelium of the vestibular system. These injections were followed by injections of the cell proliferation marker tritiated-thymidine. At predetermined intervals, the animals were killed, and the vestibular organs were processed for tissue autoradiography. Our results confirm that hair cells originate from supporting cells. The data also indicate that postmitotic cells migrate towards the lumen of the epithelium where they differentiate into Type II hair cells. At a later time, some of the new Type II hair cells further differentiate into Type I hair cells. These results suggest that both types of avian vestibular hair cells have a common ancestor. The data also provide evidence in support of the hypothesis that calyx enclosed Type I hair cells, only present in birds and mammals, are a more differentiated stage of Type II hair cells.
先前的研究表明，鸟类前庭系统的感觉上皮具有在持续基础上以及在严重损伤后替换毛细胞的能力。感觉上皮内的支持细胞被认为是再生毛细胞的祖细胞。在本研究中，我们描述了孵化后鸟类中再生前庭毛细胞形成的一系列事件。雏鸡接受硫酸链霉素注射以损伤前庭系统的感觉上皮。这些注射之后再注射细胞增殖标记物氚标记胸腺嘧啶核苷。在预定的时间间隔，处死动物，并对前庭器官进行组织放射自显影处理。我们的结果证实毛细胞起源于支持细胞。数据还表明，有丝分裂后的细胞朝着上皮腔迁移，在那里它们分化为II型毛细胞。在稍后的时间，一些新的II型毛细胞进一步分化为I型毛细胞。这些结果表明，两种类型的鸟类前庭毛细胞有一个共同的祖先。数据还为以下假说提供了支持证据，即仅存在于鸟类和哺乳动物中的杯状包绕的I型毛细胞是II型毛细胞的一个更分化的阶段。
