Pathak Chander Mohan, Avti Pramod Kumar, Kumar Surender, Khanduja Krishan Lal, Sharma Suresh Chander
Postgraduate Institute of Medical Education and Research, Chandigarh, India.
J Radiat Res. 2007 Mar;48(2):113-20. doi: 10.1269/jrr.06063. Epub 2007 Mar 6.
We examined the effect of whole body low-dose gamma-irradiation on the status of the antioxidant defense system in the rodent kidneys at different time intervals. Young male Balb/c mice were exposed to whole body radiation from a (60)Co source at doses of 10, 25 and 50 cGy (48.78 cGy/min). Antioxidant status and lipid peroxidation were estimated in the kidneys at 4, 12 and 24 h after irradiation. Lipid peroxidation increased between 33% and 49% and reduced glutathione between 12% and 47% at 12 h at different radiation doses. Reduced glutathione level remained significantly (p < 0.05) elevated even at 24 h after irradiation to 25 cGy. Superoxide dismutase activity also increased by 37% at 12 h on exposure of animals to all the doses up to 50 cGy. Catalase activity increased significantly at 12 h on exposure to 10 cGy and 50 cGy. Interestingly, glutathione peroxidase activity increased by 31% at 4 h and subsequently returned to control levels at 24 h after exposure to 50 cGy. Glutathione reductase activity increased by 10-12% at 12 h after exposure to 25 cGy and 50 cGy. The results suggest that the whole body exposure of animals to gamma radiation stimulates the antioxidant defense system in the kidneys within 4 to 24 h after irradiation, at doses of 25 cGy and 50 cGy.
我们研究了全身低剂量γ射线照射在不同时间间隔对啮齿动物肾脏抗氧化防御系统状态的影响。将年轻雄性Balb/c小鼠暴露于来自（60）Co源的全身辐射，剂量分别为10、25和50 cGy（48.78 cGy/分钟）。在照射后4、12和24小时评估肾脏中的抗氧化状态和脂质过氧化。在不同辐射剂量下，12小时时脂质过氧化增加了33%至49%，还原型谷胱甘肽减少了12%至47%。即使在照射25 cGy后24小时，还原型谷胱甘肽水平仍显著（p < 0.05）升高。在动物暴露于高达50 cGy的所有剂量下，12小时时超氧化物歧化酶活性也增加了37%。暴露于10 cGy和50 cGy时，12小时过氧化氢酶活性显著增加。有趣的是，暴露于50 cGy后4小时，谷胱甘肽过氧化物酶活性增加了31%，随后在24小时恢复到对照水平。暴露于25 cGy和50 cGy后12小时，谷胱甘肽还原酶活性增加了10%至12%。结果表明，动物全身暴露于γ射线辐射后，在25 cGy和50 cGy剂量下，照射后4至24小时内可刺激肾脏中的抗氧化防御系统。
