金鸡纳愈伤组织培养物中生物碱和蒽醌类化合物的鉴定；植物生长调节剂和光照对生物碱含量的影响。

Identification of Alkaloids and Anthraquinones in Cinchona pubescens Callus Cultures; the Effect of Plant growth Regulators and Light on the Alkaloid Content.

机构信息

Department of Pharmacognosy, State University of Leiden, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands.

出版信息

Planta Med. 1984 Feb;50(1):17-20. doi: 10.1055/s-2007-969609.

DOI:10.1055/s-2007-969609

PMID:17340239

Abstract

Studies were performed on the alkaloid content of leaf and stem callus cultures of CINCHONA PUBESCENS Vahl with respect to the influence of plant growth regulators and light. The same alkaloids were found in the analysed tissues - with some quantitative differences. In general, a medium containing zeatin: 1 microM and 2,4-dichlorophenoxyacetic acid: 1 microM gave a higher alkaloid production than a medium containing benzyladenine: 8.1 microM and 2,4-dichlorophentoxy acetic acid: 4.5 microM. The alkaloid production in the unorganized callus cultures was stimulated by light. Anthraquinones were found in all tissue cultures.

摘要

研究了金鸡纳（CINCHONA PUBESCENS Vahl）叶和茎愈伤组织的生物碱含量，以研究植物生长调节剂和光照的影响。在分析的组织中发现了相同的生物碱，只是含量有所不同。一般来说，含有 1μM 的玉米素和 1μM 的 2,4-二氯苯氧乙酸的培养基比含有 8.1μM 的苄基腺嘌呤和 4.5μM 的 2,4-二氯苯氧乙酸的培养基产生更高的生物碱产量。在无组织的愈伤组织培养中，光照可以刺激生物碱的产生。蒽醌类化合物在所有组织培养物中均有发现。