Fulzele D P, Satdive R K, Pol B B
Plant Biotechnology and Secondary Products Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
Planta Med. 2001 Mar;67(2):150-2. doi: 10.1055/s-2001-11519.
Callus cultures were initiated from stem parts of Nothapodytes foetida on Murashige and Skoog's medium supplemented with different growth regulators. Suspension cultures were established and the cell biomass was higher in the presence of NAA in comparison with 2,4-D. Culture medium supplemented with NAA (10.74 microM) and BA (2.22 microM) attained 31.3 g/l DW during 20 days of cultivation in shake flasks. In the presence of NAA, maximum concentrations of camptothecin (0.035 mg/ml) and 9-methoxycamptothecin (0.026 mg/ml) were found in the medium. Alkaloid production was reduced in presence of 2,4-D in the culture medium. Cells contained trace amount of alkaloids. Alkaloids were detected and identified by means of TLC and HPLC.
从臭豆树的茎段在添加了不同生长调节剂的Murashige和Skoog培养基上诱导出愈伤组织培养物。建立了悬浮培养物，与2,4 - D相比，在萘乙酸（NAA）存在下细胞生物量更高。添加了NAA（10.74微摩尔）和苄氨基腺嘌呤（BA，2.22微摩尔）的培养基在摇瓶中培养20天时达到了31.3克/升干重。在NAA存在下，培养基中发现了最高浓度的喜树碱（0.035毫克/毫升）和9 - 甲氧基喜树碱（0.026毫克/毫升）。培养基中存在2,4 - D时生物碱产量降低。细胞含有微量生物碱。通过薄层色谱法（TLC）和高效液相色谱法（HPLC）检测和鉴定生物碱。
