Roth T L, White K L, Thompson D L, Horohov D W
Department of Animal Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge.
J Reprod Immunol. 1992 Jan;21(1):87-95. doi: 10.1016/0165-0378(92)90042-3.
It has been proposed that PGE2 is an important immunosuppressant acting at the fetal-maternal interface during pregnancy. We have previously shown that horse conceptus-conditioned medium suppresses lymphocyte proliferation. This experiment was designed to determine if horse conceptus-derived immunosuppressive activity could be attributed to PGE2 production by the trophoblast tissue. Trophoblast tissue from 21-day-old conceptuses was cut into equal sections and cultured in the presence or absence of the prostaglandin inhibitor, indomethacin. Following culture, immunosuppressive activity and the concentration of PGE2 were determined for each sample of both horse-trophoblast conditioned medium (HTCM) and indomethacin-treated HTCM (I-HTCM). Suppressive activity was identified in lymphocyte proliferation assays via reduced [3H]thymidine uptake by pokeweed mitogen stimulated horse lymphocytes. A radioimmunoassay was used to quantify PGE2. While PGE2 production was greatly reduced in cultures containing indomethacin, trophoblast-derived immunosuppressive activity was not affected. These data indicate that PGE2 is not the primary immunosuppressant produced by horse trophoblast tissue.
有人提出，前列腺素E2（PGE2）是孕期作用于胎儿-母体界面的一种重要免疫抑制剂。我们之前已经表明，马孕体条件培养基可抑制淋巴细胞增殖。本实验旨在确定马孕体来源的免疫抑制活性是否可归因于滋养层组织产生的PGE2。将21日龄孕体的滋养层组织切成相等的切片，在存在或不存在前列腺素抑制剂吲哚美辛的情况下进行培养。培养后，测定马滋养层条件培养基（HTCM）和吲哚美辛处理的HTCM（I-HTCM）每个样品的免疫抑制活性和PGE2浓度。通过商陆有丝分裂原刺激的马淋巴细胞对[3H]胸腺嘧啶核苷摄取减少，在淋巴细胞增殖试验中鉴定抑制活性。采用放射免疫分析法对PGE2进行定量。虽然在含有吲哚美辛的培养物中PGE2的产生大大减少，但滋养层来源的免疫抑制活性不受影响。这些数据表明，PGE2不是马滋养层组织产生的主要免疫抑制剂。
