Bolder Suzanne G, Sagis Leonard M C, Venema Paul, van der Linden Erik
DMV International b.v., P.O. Box 13, 5460 BA Veghel, The Netherlands.
Langmuir. 2007 Apr 10;23(8):4144-7. doi: 10.1021/la063048k. Epub 2007 Mar 7.
The conversion of protein monomers into fibrils can be determined using the centrifugal filtration method. The results of this method were used to calibrate steady-shear birefringence and Thioflavin T fluorescence measurements. For both measurements, a linear correlation with the fibril concentration was extracted, resulting in two fast assays to determine the fibril concentration quantitatively. From birefringence measurements and the conversion determined using the centrifugal filtration method, we were able to calculate more precise values for the birefringence per unit length of the fibrils (M) and the flexibility of the fibrils (beta).
蛋白质单体向纤维的转化可以通过离心过滤法来测定。该方法的结果用于校准稳态剪切双折射和硫黄素T荧光测量。对于这两种测量，都提取了与纤维浓度的线性相关性，从而得到两种快速测定方法来定量测定纤维浓度。通过双折射测量和使用离心过滤法测定的转化率，我们能够计算出纤维每单位长度的双折射（M）和纤维的柔韧性（β）的更精确值。
