Cheremisina Z P, Vladimirov A
Biokhimiia. 1975 Mar-Apr;40(2):242-7.
Effect of Fe2+- and ascorbate-induced lipid peroxidation on mitochondrial respiration in 3 and 4 states (according to Chance) is studied. Peroxidation was shown to result in an increase of the oxygen uptake rate in 4th state, and in the KCl-containing medium (but not in sucrose medium) it caused a decrease of the oxygen uptake rate in the phosphorylating state, which partially reversed when cytochrom c was added. These effects were observed only after the development of peroxidation (after "slow" flash of chemiluminescence), and the value of and effect correlated with the content of peroxidation products unsaturated fatty acids. No considerable differences in the damaging effect of peroxidation were observed under incubation of mitochondria in KCl, NaCl and choline containing media.
研究了Fe2+和抗坏血酸盐诱导的脂质过氧化对处于3态和4态（根据钱斯的定义）线粒体呼吸的影响。结果表明，过氧化导致第4态氧摄取率增加，在含KCl的介质中（但在蔗糖介质中未出现这种情况），它会导致磷酸化状态下氧摄取率降低，添加细胞色素c后这种降低会部分逆转。这些效应只有在过氧化发展之后（化学发光“缓慢”闪烁之后）才会观察到，效应值与过氧化产物不饱和脂肪酸的含量相关。在KCl、NaCl和含胆碱的介质中孵育线粒体时，未观察到过氧化损伤效应有显著差异。
