Laboratory of Toxicology, Department of Toxicology and Pharmacology, Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Laboratory of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center and Laboratory of Histopathology, School of Medicine, Tehran University of Medical Sciences Tehran, Iran.
Evid Based Complement Alternat Med. 2007 Mar;4(1):43-50. doi: 10.1093/ecam/nel051. Epub 2006 Sep 14.
Inflammatory bowel disease (IBD) is a chronic condition of the intestine with unknown etiology involving multiple immune, genetic and environmental factors. We were interested to examine the effect of total extract from Zataria multiflora Boiss, a folk medicinal plant on prevention and treatment of experimental IBD. Z. multiflora was administered (400, 600, 900 p.p.m.) through drinking water to IBD mice induced by intrarectal administration of acetic acid. Prednisolone was used as the standard drug for comparison. Biochemical, macroscopic and microscopic examinations of colon were performed. Biochemical evaluation of inflamed colon was done using assay of myeloperoxidase (MPO) activity and thiobarbituric acid reactive substances (TBARS) concentration as indicators of free radical activity and cell lipid peroxidation. The activity of MPO and lipid peroxidation products (TBARS) increased in acetic acid-treated groups while recovered by pretreatment of animals with Z. multiflora (400-900 p.p.m.) and prednisolone. Z. multiflora (600 and 900 p.p.m.) and prednisolone-treated groups showed significantly lower score values of macroscopic and microscopic characters when compared with the acetic acid-treated group. The beneficial effect of Z. multiflora (900 p.p.m.) was comparable with that of prednisolone. The antioxidant, antimicrobial and anti-inflammatory potentials of Z. multiflora might be the mechanisms by which this herbal extract protects animals against experimentally induced IBD. Proper clinical investigation should be carried out to confirm the activity in human.
炎症性肠病（IBD）是一种慢性肠道疾病，病因不明，涉及多种免疫、遗传和环境因素。我们有兴趣研究泽兰多花香草总提取物对实验性 IBD 的预防和治疗作用。泽兰多花香草通过饮用水给药（400、600、900ppm），用于经直肠内给予乙酸诱导的 IBD 小鼠。泼尼松龙被用作比较的标准药物。对结肠进行生化、宏观和微观检查。通过测定髓过氧化物酶（MPO）活性和硫代巴比妥酸反应物质（TBARS）浓度作为自由基活性和细胞脂质过氧化的指标，对发炎结肠进行生化评估。在乙酸处理组中，MPO 和脂质过氧化产物（TBARS）的活性增加，而用泽兰多花香草（400-900ppm）和泼尼松龙预处理动物后则恢复。与乙酸处理组相比，泽兰多花香草（600 和 900ppm）和泼尼松龙处理组的宏观和微观特征评分值显著降低。泽兰多花香草（900ppm）的有益作用与泼尼松龙相当。泽兰多花香草的抗氧化、抗菌和抗炎潜力可能是这种草药提取物保护动物免受实验性 IBD 侵害的机制。应进行适当的临床研究以确认其在人类中的活性。
