Berman A E
Biokhimiia. 1975 Mar-Apr;40(2):432-40.
Synthesys of collagen and non-collagen proteins was investigated in a cell-free system in the presence of free and bound polysomes isolated from chick embryos. Of total radioactive proteins synthesized on bound and free polysomes the amount of peptides digested by bacterial collagenase comprised 25-40% and 5-7% respectively. These data showed that collagen was predominantly synthesized by bound polysomes. Free polysomes were found to be much more active than bound ones in non-collagen protein synthesis. When bound polysomes detached from membranes by detergent treatment were incubated in a cell-free system, a release of non-collagen proteins into the incubation medium increased sharply, but the release of collagen peptides was as negligible as in the case of untreated polysomes. Kinetic studies of collagen synthetizing activity of polysomes bound to or detached from membranes suggested the role of endoplasmic membranes in stabilizing collagenous polysomes.
在存在从鸡胚中分离出的游离和结合多核糖体的无细胞系统中，研究了胶原蛋白和非胶原蛋白的合成。在结合和游离多核糖体上合成的总放射性蛋白质中，被细菌胶原酶消化的肽量分别占25%-40%和5%-7%。这些数据表明，胶原蛋白主要由结合多核糖体合成。发现游离多核糖体在非胶原蛋白合成中比结合多核糖体活跃得多。当通过去污剂处理使从膜上分离的结合多核糖体在无细胞系统中孵育时，非胶原蛋白释放到孵育培养基中的量急剧增加，但胶原肽的释放与未处理的多核糖体一样可忽略不计。对与膜结合或从膜上分离的多核糖体的胶原合成活性的动力学研究表明内质网在稳定胶原多核糖体方面的作用。
