Belkin V M, Volodarskaia S M, Mazurov V I
Biokhimiia. 1985 Dec;50(12):2003-9.
Using immunochemical methods, the fibronectin-synthesizing activity of membrane-bound and free polyribosomes in a cell-free system was studied. It was demonstrated that fibronectin biosynthesis on membrane-bound polyribosomes from human embryonic fibroblasts makes up to 4.9%, while that from chicken embryos--1.1% of the total amount of the de novo synthesized proteins (as compared to 1.0 and 0.3% in free polyribosomes, respectively). Fibronectin monomers (Mr = 330 kD) were detected only in the newly synthesized (in the presence of spermidine) material of the cell-free system containing heavy fractions of membrane-bound polyribosomes.
运用免疫化学方法，对无细胞体系中膜结合型和游离型多核糖体的纤连蛋白合成活性进行了研究。结果表明，人胚胎成纤维细胞膜结合型多核糖体上的纤连蛋白生物合成量占从头合成蛋白质总量的4.9%，而鸡胚胎的该比例为1.1%（相比之下，游离型多核糖体中的比例分别为1.0%和0.3%）。仅在含有膜结合型多核糖体重组分的无细胞体系新合成（在亚精胺存在下）物质中检测到了纤连蛋白单体（Mr = 330 kD）。
