人类通用转录因子TFIIE小亚基中的保守序列基序。

Conserved sequence motifs in the small subunit of human general transcription factor TFIIE.

作者信息

Sumimoto H, Ohkuma Y, Sinn E, Kato H, Shimasaki S, Horikoshi M, Roeder R G

机构信息

Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.

出版信息

Nature. 1991 Dec 5;354(6352):401-4. doi: 10.1038/354401a0.

DOI:10.1038/354401a0

PMID:1956404

Abstract

A general initiation factor, TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57,000 (TFIIE-alpha) and two of 34,000 (TFIIE-beta). TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. Here we report the cloning and expression of a complementary DNA encoding a functional human TFIIE-beta. Recombinant TFIIE-beta could replace the natural TFIIE-beta for transcription in conjunction with TFIIE-alpha. Amino-acid sequence comparisons reveal regions with sequence similarities to: subregion 3 of bacterial sigma factors; a region of RAP30 (the small subunit of TFIIF) with sequence similarity to a sigma-factor subregion implicated in binding to RNA polymerase; and a portion of the basic region-helix-loop-helix motif found in several enhancer-binding proteins. These potential homologies have implications for the role of TFIIE in preinitiation complex assembly and function.

摘要

一种通用起始因子TFIIE，对于RNA聚合酶II与其他通用因子共同进行转录起始至关重要。TFIIE是一种异源四聚体，包含两个相对分子质量为57,000的亚基（TFIIE-α）和两个34,000的亚基（TFIIE-β）。TFIIE-β与TFIIE-α共同参与转录起始。在此我们报道了编码功能性人TFIIE-β的互补DNA的克隆与表达。重组TFIIE-β可与TFIIE-α共同取代天然TFIIE-β进行转录。氨基酸序列比较揭示了与以下区域具有序列相似性的区域：细菌σ因子的亚区域3；RAP30（TFIIF的小亚基）中与涉及结合RNA聚合酶的σ因子亚区域具有序列相似性的一个区域；以及在几种增强子结合蛋白中发现的碱性区域-螺旋-环-螺旋基序的一部分。这些潜在的同源性对TFIIE在起始前复合物组装和功能中的作用具有启示意义。