Lengyel G, Joachim B, Acs G, Fehér J
Semmelweis Orvostudományi Egyetem, II. Belklinika, Budapest.
Orv Hetil. 1992 Jan 5;133(1):11-5.
The authors studied the function of human fetal liver cell--on level of DNA and gene regulation--by methods of molecular biology. Experiments were done under strict observation of the ethical guidelines of the 1975 Declaration of Helsinki Human Research Committee. They conclude that human fetal liver culture may serve as a suitable in vitro modell for study of the liver specific gene expressions. They found that cultured human fetal liver cells from second trimester express albumin and AFP. They demonstrate that fetal hepatocytes--like adult hepatocytes--respond to the inflammatory mediators, IL-1, IL-6 and TNF, by induction of CRP and alpha-1-Ach expression and regression of albumin synthesis proving the ability of fetal hepatocytes to produce an acute phase response.
作者们通过分子生物学方法，在DNA水平和基因调控层面研究了人类胎儿肝细胞的功能。实验严格遵循了1975年《赫尔辛基宣言》人类研究委员会的伦理准则。他们得出结论，人类胎儿肝脏培养物可作为研究肝脏特异性基因表达的合适体外模型。他们发现，孕中期培养的人类胎儿肝细胞可表达白蛋白和甲胎蛋白。他们证明，胎儿肝细胞——与成人肝细胞一样——通过诱导C反应蛋白和α-1-抗胰蛋白酶表达以及白蛋白合成减少来对炎症介质白细胞介素-1、白细胞介素-6和肿瘤坏死因子作出反应，这证明了胎儿肝细胞产生急性期反应的能力。
