Lamacchia Celine, Palmer Gaby, Gabay Cem
Department of Pathology and Immunology, University of Geneva School of Mediicne, and Department of Internal Medicine, University Hospital, Switzerland.
J Am Assoc Lab Anim Sci. 2007 Mar;46(2):21-4.
We present a list of simple sequence length polymorphisms (SSLPs) that was used in a polymerase chain reaction (PCR)-based marker-assisted selection protocol to facilitate the generation of a C57BL/6J Rj congenic strain from a mixed 129S2/SvPasCrlxC57BL/6J Rj background. We chose informative SSLP markers that would permit evaluation of the PCR products on NuSieve agarose or nondenaturing polyacrylamide gels. This list of oligonucleotide pairs is useful for analyzing and backcrossing knockout or transgenic strains derived from 129S2/SvPasCrl or C57BL/6J Rj backgrounds.
我们列出了一系列简单序列长度多态性（SSLP），这些SSLP用于基于聚合酶链反应（PCR）的标记辅助选择方案，以促进从混合的129S2/SvPasCrlx C57BL/6J Rj背景中培育出C57BL/6J Rj同基因系。我们选择了信息丰富的SSLP标记，以便能够在NuSieve琼脂糖或非变性聚丙烯酰胺凝胶上评估PCR产物。这份寡核苷酸对列表有助于分析和回交源自129S2/SvPasCrl或C57BL/6J Rj背景的基因敲除或转基因品系。
