Holden Lise, Moen Lena Haugland, Sletten Gaynour B G, Dooper Maaike M B W
National Veterinary Institute, P.O. Box 8156 Dep., N-0033 Oslo, Norway.
J Agric Food Chem. 2007 Apr 4;55(7):2536-42. doi: 10.1021/jf063320w. Epub 2007 Mar 8.
Sweet lupines are increasingly used in food production. Cause for concern has been expressed due to the increase in reported lupine-induced allergic incidents and the association between lupine and peanut allergies. In the current study, a polyclonal-monoclonal antibody-based sandwich ELISA for the detection of lupine proteins in foods was developed. The assay was sensitive to both native and processed proteins from Lupinus angustifolius and Lupinus albus and had a detection limit of 1 mug/g. Intra- and interassay coefficients of variation were <5 and <17%, respectively. A selection of 112 food samples, both with and without lupine declaration, was evaluated for their content of lupine. The data showed that the majority were in agreement with the respective labeling. However, some inconsistency was seen, typically in bread/rolls and soy flours.
甜羽扇豆在食品生产中的应用日益广泛。由于报告的羽扇豆诱发过敏事件增多以及羽扇豆与花生过敏之间的关联，人们对此表示担忧。在本研究中，开发了一种基于多克隆 - 单克隆抗体的夹心酶联免疫吸附测定法（ELISA），用于检测食品中的羽扇豆蛋白。该测定法对窄叶羽扇豆和白羽扇豆的天然蛋白和加工蛋白均敏感，检测限为1微克/克。批内和批间变异系数分别<5%和<17%。对112份有或没有羽扇豆声明的食品样本进行了羽扇豆含量评估。数据显示，大多数样本与各自的标签一致。然而，也发现了一些不一致的情况，典型的是在面包/卷和大豆粉中。
