新生儿和小儿幽门螺杆菌粪便抗原的检出率

Detection rate of Helicobacter pylori stool antigen in newborn infants and small children.

作者信息

Stray-Pedersen Arne, Gaustad Peter, Stray-Pedersen Babill, Rognum Torleiv O

机构信息

Institute of Forensic Medicine, University of Oslo, Norway.

出版信息

J Perinat Med. 2007;35(2):155-8. doi: 10.1515/JPM.2007.040.

DOI:10.1515/JPM.2007.040

PMID:17343545

Abstract

AIMS

To investigate the prevalence of H. pylori antigen in the stools of Norwegian neonates and small children.

METHODS

A total of 249 children aged 0 days-3 years were tested for the presence of H. pylori antigen in feces using the HpSA immunoassay. For verification purposes, a selection of samples were analyzed with PCR targeting the 16 S rDNA Helicobacter gene.

RESULTS

H. pylori antigen in stool was detected in 52% (36/69) of the neonates, in 15% (7/46) of infants aged 7 days-1 month, and in 5% (7/134) of children aged 1 month-3 years. In neonates, H. pylori antigen detection was significantly associated with mode of delivery: 59% (30/51) with uncomplicated vaginal births were HpSA positive compared to only 10% (1/10) of infants delivered by cesarean section (P=0.02). Positive PCR results were found in 35% (9/26) of HpSA positive samples. Sequencing of PCR products revealed 97-100% homology with gene sequences from both H. pylori and other Helicobacter species.

CONCLUSIONS

The low H. pylori antigen detection rate in children >1 month of age is in accordance with previous prevalence studies from Western countries. The unexpected finding of a high H. pylori antigen detection rate in neonates suggests that transient colonization may occur in the neonatal period.

摘要

目的

调查挪威新生儿和幼儿粪便中幽门螺杆菌抗原的流行情况。

方法

使用HpSA免疫分析法对总共249名年龄在0天至3岁的儿童进行粪便中幽门螺杆菌抗原检测。为进行验证，选取部分样本采用靶向16S rDNA幽门螺杆菌基因的PCR进行分析。

结果

在新生儿中，52%（36/69）检测到粪便中有幽门螺杆菌抗原；7日龄至1月龄婴儿中，15%（7/46）检测到；1月龄至3岁儿童中，5%（7/134）检测到。在新生儿中，幽门螺杆菌抗原检测与分娩方式显著相关：顺产无并发症的婴儿中59%（30/51）HpSA呈阳性，而剖宫产婴儿中只有10%（1/10）呈阳性（P = 0.02）。在HpSA呈阳性的样本中，35%（9/26）PCR结果为阳性。PCR产物测序显示与幽门螺杆菌和其他螺杆菌属的基因序列有97 - 100%的同源性。