构象调整和远程静电力对CD2/CD58相互作用的贡献。
The contribution of conformational adjustments and long-range electrostatic forces to the CD2/CD58 interaction.
作者信息
Kearney Alice, Avramovic Adam, Castro Mónica A A, Carmo Alexandre M, Davis Simon J, van der Merwe P Anton
机构信息
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
出版信息
J Biol Chem. 2007 May 4;282(18):13160-6. doi: 10.1074/jbc.M700829200. Epub 2007 Mar 7.
Abstract
CD2 is a T cell surface molecule that enhances T and natural killer cell function by binding its ligands CD58 (humans) and CD48 (rodents) on antigen-presenting or target cells. Here we show that the CD2/CD58 interaction is enthalpically driven and accompanied by unfavorable entropic changes. Taken together with structural studies, this indicates that binding is accompanied by energetically significant conformational adjustments. Despite having a highly charged binding interface, neither the affinity nor the rate constants of the CD2/CD58 interaction were affected by changes in ionic strength, indicating that long-range electrostatic forces make no net contribution to binding.
摘要
CD2是一种T细胞表面分子，它通过与抗原呈递细胞或靶细胞上的配体CD58（人类）和CD48（啮齿动物）结合来增强T细胞和自然杀伤细胞的功能。我们在此表明，CD2/CD58相互作用是由焓驱动的，并且伴随着不利的熵变。结合结构研究，这表明结合伴随着能量上显著的构象调整。尽管具有高电荷的结合界面，但CD2/CD58相互作用的亲和力和速率常数均不受离子强度变化的影响，这表明长程静电力对结合没有净贡献。
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