Department of Plant Genetics, The Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel.
Planta Med. 1985 Dec;51(6):511-4. doi: 10.1055/s-2007-969578.
Exposure of MENTHA cells to 500 Gy gamma-irradiation caused cell-division arrest. These irradiated cells efficiently reduced (-)menthone to (+)neomenthol with similar or higher activity than that of the corresponding non-irradiated cells. Moreover the menthone reduction capability of the irradiated cells was fully retained following immobilization by entrapment in glyoxal-crosslinked polyacrylamide-hydrazide. Gamma-irradiated cells retained their biotransformation capability for at least one week in shake-culture; thus the transformation process could be repeated several times. Efficient biotransformation carried out by irradiated plant cells seems to be a general phenomenon. In addition to the capability of irradiated MENTHA cells to reduce either (-)menthone or another precursor (geraniol), cells derived from NICOTIANA SYLVESTRIS also retained their capacity of biotransformation, after gamma-irradiation. Division-arrested and immobilized plant cells may thus allow the development of continuous biotransformation processes.
MENTHA 细胞暴露在 500Gy 的γ射线辐射下会导致细胞分裂停滞。这些辐照后的细胞能有效地将（-）薄荷酮还原为（+）新薄荷醇，其活性与相应的未辐照细胞相似或更高。此外，辐照后的细胞通过与乙二醛交联的聚丙烯酰胺-酰肼包埋固定化后，其薄荷酮还原能力得以完全保留。在摇瓶培养中，辐照细胞至少能保持一周的生物转化能力；因此，该转化过程可以重复多次。辐照植物细胞的高效生物转化似乎是一种普遍现象。除了辐照 MENTHA 细胞还原（-）薄荷酮或另一种前体（香叶醇）的能力外，经过γ射线辐照后，来自 NICOTIANA SYLVESTRIS 的细胞也保留了它们的生物转化能力。因此，被分裂抑制和固定化的植物细胞可以实现连续的生物转化过程。
