Evidence for 16alpha-hydroxylation of estrone 3-sulphate by guinea pig liver slices.
作者信息
Hobkirk R, Nilsen M, Jennings B
出版信息
Can J Biochem. 1975 Oct;53(10):1133-5. doi: 10.1139/o75-157.
DOI:10.1139/o75-157
PMID:173453
Abstract
[6,7-3H,35S]Estrone 3-sulfate (E13S) of 3H/35S = 3.57 was incubated with female guinea pig liver slices. Small amounts of free steroid and estrone-3-glucuronide, each containing 3H, were found. In addition, E13S, 17beta-estradiol 3-sulfate, and a 'disulfate' fraction, with 3H/35S = 4.4, 4.3, and 4.7, respectively, were also isolated from the incubated tissue. The latter fraction was a major metabolite and about 45% of it consisted of 'disulfates' of 16alpha-hydroxyestrone and estriol, thus providing strong evidence for 16alpha-hydroxylation in guinea pig liver slices.
摘要
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引用本文的文献
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A reliable radiochromatographic assay technique for hepatic microsomal 16 alpha-hydroxylase activity towards oestrone 3-sulphate. Comparison between pigmented and non-pigmented mature guinea pigs.一种用于检测肝微粒体对硫酸雌酮3的16α-羟化酶活性的可靠放射色谱分析技术。有色和无色成年豚鼠之间的比较。
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