Kaneko Y, Iwaki T, Fukui M
Department of Neuropathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Arch Pathol Lab Med. 1992 Jan;116(1):60-4.
Lectin histochemical studies of human fetal notochord, ecchordosis physaliphora, and eight chordomas were performed. Ecchordosis physaliphora and eight chordomas were stained with Ricinus communis type I, Canavalia ensiformis, Triticum vulgaris, and Limax flavus. Ricinus communis type I, T vulgaris, and L flavus reacted with both tumor cells and the extracellular mucinous matrix, while C ensiformis mainly reacted with the cytoplasm of tumor cells. Thus, tumor cells were most recognizable with the C ensiformis stain. After neuraminidase treatment, ecchordosis physaliphora and chordomas invariably showed positivities for Arachis hypogaea. The lectin-binding patterns of chordomas closely reflected those of the human fetal notochord. Chordomas were completely sialylated regardless of either the clinical course or histopathological findings. Among the eight lectins, C ensiformis heavily labeled chordoma cells but not extracellular space. This was probably because asparagine-linked N-glycosylation was active in the chordomas, while the high-mannose-type oligosaccharides were apt to remain in the cytoplasm.
对人胎儿脊索、泡状脊索瘤及8例脊索瘤进行了凝集素组织化学研究。泡状脊索瘤及8例脊索瘤用I型蓖麻凝集素、刀豆、小麦胚芽凝集素及黄蛞蝓凝集素进行染色。I型蓖麻凝集素、小麦胚芽凝集素及黄蛞蝓凝集素与肿瘤细胞及细胞外黏液基质均发生反应，而刀豆主要与肿瘤细胞的细胞质发生反应。因此，肿瘤细胞在刀豆染色时最易辨认。经神经氨酸酶处理后，泡状脊索瘤和脊索瘤对花生凝集素均呈阳性反应。脊索瘤的凝集素结合模式与人类胎儿脊索的模式密切相关。无论临床病程或组织病理学表现如何，脊索瘤均完全被唾液酸化。在这8种凝集素中，刀豆对脊索瘤细胞的标记程度很高，但对细胞外间隙无标记。这可能是因为天冬酰胺连接的N-糖基化在脊索瘤中很活跃，而高甘露糖型寡糖易于保留在细胞质中。
