Filella Xavier, Alcover Joan, Molina Rafael, Luque Pilar, Corral Juan M, Augé Josep M, Coca Francisca
Department of Biochemistry and Molecular Genetics, Hospital Clinic, Barcelona, Catalonia, Spain.
Anticancer Res. 2007 Jan-Feb;27(1B):607-10.
Free prostate-specific antigen (fPSA), the minor form of total PSA, contains different molecular subforms, including BPSA and proPSA. Whereas BPSA is associated with benign prostate hyperplasia, proPSA is associated with prostate tumor.
The serum levels of PSA, fPSA and proPSA were measured using automated electrochemiluminescent immunoassays (Elecsys 2010, Roche Diagnostics) in 87 patients with prostate cancer and 138 patients with benign prostate hyperplasia. Also, we calculated the derived tests of these assays through the subtraction or the ratio between the measured tests.
Receiver operating characteristics curves were used for comparison of the diagnostic utility of tests assessed. The biggest areas were obtained for the free/total PSA ratio (0.705), the calculated Bfree PSA/total PSA ratio (0.719) and the calculated Bfree PSA/bound PSA ratio (0. 726).
Applying a multivariate logistic regression analysis, it was determined that the combination of the proPSA concentration, the proPSA/total PSA ratio and the calculated Bfreeltotal PSA ratio improves the area under the curve obtained for individual tests (0.753). ProPSA may be useful in the diagnosis of prostate cancer.
游离前列腺特异性抗原（fPSA）是总PSA的次要形式，包含不同的分子亚形式，包括BPSA和proPSA。BPSA与良性前列腺增生相关，而proPSA与前列腺肿瘤相关。
使用自动化电化学发光免疫分析法（Elecsys 2010，罗氏诊断公司）测定了87例前列腺癌患者和138例良性前列腺增生患者的血清PSA、fPSA和proPSA水平。此外，我们通过测量值之间的减法或比值计算了这些检测的衍生指标。
采用受试者工作特征曲线比较所评估检测的诊断效用。游离/总PSA比值（0.705）、计算得到的B游离PSA/总PSA比值（0.719）和计算得到的B游离PSA/结合PSA比值（0.726）的曲线下面积最大。
应用多因素逻辑回归分析确定，proPSA浓度、proPSA/总PSA比值与计算得到的B游离/总PSA比值的组合可提高各单项检测的曲线下面积（0.753）。ProPSA可能有助于前列腺癌的诊断。
