一种d(ApG)铂化九核苷酸双链体的合成与表征

Synthesis and characterization of a d(ApG) platinated nonanucleotide duplex.

作者信息

Fouchet M H, Gauthier C, Guittet E, Girault J P, Igolen J, Chottard J C

机构信息

Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, URA 400 CNRS, Université René Descartes, Paris, France.

出版信息

Biochem Biophys Res Commun. 1992 Jan 31;182(2):555-60. doi: 10.1016/0006-291x(92)91768-l.

DOI:10.1016/0006-291x(92)91768-l

PMID:1734870

Abstract

The nonamer 5'd(CTCAGCCTC) 3' 1 has been reacted with cis-diamminediaquaplatinum(II) in water at pH 4.2. The major reaction product was shown by enzymatic digestion and 1H NMR to be the d(ApG)cis-Pt(NH3)2 chelate [cis-Pt(NH3)2[d(CTCAGCCTC)-N7(4),N7(5)]] 1-Pt. When mixed with its complementary strand 2, 1-Pt forms a B DNA type duplex 3-Pt with a Tm of 35 degrees C (versus 58 degrees C for the unplatinated duplex). The NMR study of the exchangeable protons of 3-Pt revealed that the helix distortion is localized on the CAG-CTG moiety (the asterisks indicating the platinum chelation sites) with a strong perturbation of the A*(4)T(15) base pair related to a large tilt of A*(4).

摘要

九聚体5'd(CTCAGCCTC) 3' 1已在pH值为4.2的水中与顺二氨二水合铂(II)发生反应。酶切和1H NMR分析表明，主要反应产物是d(ApG)顺铂(NH3)2螯合物[顺铂(NH3)2[d(CTCAGCCTC)-N7(4),N7(5)]] 1-Pt。当1-Pt与其互补链2混合时，会形成B型DNA双链体3-Pt，其解链温度（Tm）为35℃（未铂化双链体的Tm为58℃）。对3-Pt可交换质子的NMR研究表明，螺旋扭曲定位于CAG-CTG部分（星号表示铂螯合位点），与A*(4)的大倾斜相关的A*(4)T(15)碱基对受到强烈扰动。