一种十核苷酸双链体的高熔点顺式-[Pt(NH₃)₂[d(GpG)]]加合物。
A high melting cis-[Pt(NH3)2[d(GpG)]]adduct of a decanucleotide duplex.
作者信息
van Hemelryck B, Guittet E, Chottard G, Girault J P, Herman F, Huynh-Dinh T, Lallemand J Y, Igolen J, Chottard J C
出版信息
Biochem Biophys Res Commun. 1986 Jul 31;138(2):758-63. doi: 10.1016/s0006-291x(86)80561-7.
The [cis-Pt(NH3)2(d(GCCGGATCGC)-N7(4), N7(5))]-d(GCGATCCGGC) duplex has been prepared with Tm = 49 degrees C (vs 58 degrees C for the unplatinated form). NMR of the ten observable imino protons supports a kinked structure with intact base pairing of the duplex on the 3'-side of the d(GpG).cis-Pt chelate (relative to the platinated strand) The modification of the B-DNA type CD spectrum, due to the platinum chelate, is comparable to that observed for the platination (at a 0.05 Pt:base ratio) of the Micrococcus Lysodeikticus DNA (72% GC).
[顺式-铂（氨）₂（d（GCCGGATCGC）-N7（4），N7（5））]-d（GCGATCCGGC）双链体已制备完成，其解链温度为49℃（未铂化形式为58℃）。对十个可观测到的亚氨基质子进行的核磁共振分析支持了一种扭结结构，该双链体在d（GpG）.顺铂螯合物（相对于铂化链）的3'侧具有完整的碱基配对。由于铂螯合物导致的B-DNA型圆二色光谱的变化，与在溶壁微球菌DNA（72%GC）的铂化（铂与碱基比例为0.05）中观察到的变化相当。
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