小鼠葡萄糖转运蛋白3（GLUT3）和人类葡萄糖转运蛋白3在大脑中的表达。
Expression of mouse-GLUT3 and human-GLUT3 glucose transporter proteins in brain.
作者信息
Maher F, Vannucci S, Takeda J, Simpson I A
机构信息
Experimental Diabetes, Metabolism and Nutrition Section, NIDDK, National Institutes of Health, Bethesda, MD 20892.
出版信息
Biochem Biophys Res Commun. 1992 Jan 31;182(2):703-11. doi: 10.1016/0006-291x(92)91789-s.
Polyclonal anti-peptide antisera were raised to the C-terminal sequence of mouse- and human-GLUT3 glucose transporter isoforms. GLUT3 protein and mRNA expression were investigated by Western blot and Northern blot assays, in a range of tissues and cell lines. Mouse-GLUT3 protein was detected only in rat brain, where it was present in most regions except adenohypophysis and pineal gland. Mouse-GLUT3 was also detected in primary cultured rat cerebellar neurons and the neuronal cell lines PC12 and NG108-15, but not in cultured astroglia. Human-GLUT3 protein was detected in CHO cells transfected with the human-GLUT3 cDNA and in human brain, but not in human placenta or fat. The expression of GLUT3 in rat brain and neurons indicates it is a major neuronal glucose transporter.
制备了针对小鼠和人类GLUT3葡萄糖转运异构体C末端序列的多克隆抗肽抗血清。通过蛋白质免疫印迹法和Northern印迹法，在一系列组织和细胞系中研究了GLUT3蛋白和mRNA的表达。仅在大鼠脑中检测到小鼠GLUT3蛋白，在除腺垂体和松果体以外的大多数区域均有表达。在原代培养的大鼠小脑神经元以及神经元细胞系PC12和NG108 - 15中也检测到了小鼠GLUT3，但在培养的星形胶质细胞中未检测到。在转染了人类GLUT3 cDNA的CHO细胞和人类脑中检测到了人类GLUT3蛋白，但在人类胎盘或脂肪中未检测到。GLUT3在大鼠脑和神经元中的表达表明它是主要的神经元葡萄糖转运体。
Zotero 插件