Schrepfer Sonja, Deuse Tobias, Lange Claudia, Katzenberg Regina, Reichenspurner Hermann, Robbins Robert C, Pelletier Marc P
Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CA 94305-5407, USA.
Stem Cells Dev. 2007 Feb;16(1):105-7. doi: 10.1089/scd.2006.0041.
Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.
间充质干细胞（MSCs）被广泛应用于实验性再生策略。由于它们能够分化为间充质谱系，因此是组织再生的潜在细胞来源。由于没有可直接用于定义MSCs的特异性抗原，关于如何分离它们尚无共识。在此，我们描述了一种使用磁性细胞分选和塑料贴壁法分离、纯化和体外扩增培养小鼠骨髓间充质干细胞的简单方案。我们进一步表明，补充细胞因子可增强间充质干细胞的增殖，而不会损害其多能性。
