Abad M, Sprecher D J, Ross P, Friendship R M, Kirkwood R N
Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, USA.
Reprod Domest Anim. 2007 Apr;42(2):149-52. doi: 10.1111/j.1439-0531.2006.00744.x.
Frozen-thawed (FT) boar sperm have a reduced fertile life, due in part to a capacitation-like status induced by cooling. Reversal of this cryocapacitation in vitro by exposure to boar seminal plasma (SP) has been demonstrated. The objective of these studies was to determine the effect of SP on the ability of FT sperm to create an oviductal sperm reservoir following artificial insemination (AI). In Experiment one, 35 pre-pubertal gilts were injected (IM) with 400 IU eCG plus 200 IU hCG to induce oestrus. At detection of oestrus, gilts were inseminated with 3 x 10(9) live sperm, either fresh (FS; n = 13), FT (n = 10), or FT supplemented with 10% v/v SP (n = 12). Gilts were killed 8 h later, their reproductive tracts recovered and the uterotubal junctions (UTJs) flushed to recover sperm. Fewer (p < 0.01) sperm were recovered following FT, compared to FS, inseminations, and there was no evident effect of SP. In Experiment two, 30 pre-pubertal gilts received IM injections of 1000 IU eCG followed by 5 mg pLH 80 h later to control time of ovulation. Gilts were inseminated with 3 x 10(9) live FS sperm (n = 6), FT sperm (n = 15) or FT sperm plus 10% SP (n = 9) at 12 h before ovulation and then sacrificed 8 h later. The UTJs were dissected and flushed for sperm recovery. Fewest (p < 0.001) sperm were recovered following FT insemination and there was no evident effect of SP. These data demonstrate that the size of the sperm reservoir is markedly reduced in gilts inseminated with FT sperm. However, the lack of effect of SP suggested that either it did not reverse cryocapacitation or that such a reversal does not impact the in vivo ability to create a sperm reservoir.
冻融（FT）公猪精子的可育寿命缩短，部分原因是冷却诱导了一种类似获能的状态。已证明通过暴露于公猪精液（SP）可在体外逆转这种低温获能。这些研究的目的是确定SP对人工授精（AI）后FT精子形成输卵管精子库能力的影响。在实验一中，给35头青春期前的后备母猪肌肉注射400IU促卵泡素（eCG）加200IU人绒毛膜促性腺激素（hCG）以诱导发情。在检测到发情时，给后备母猪分别输精3×10⁹个活精子，其中新鲜精子（FS；n = 13）、FT精子（n = 10）或添加10%（v/v）SP的FT精子（n = 12）。8小时后处死后备母猪，回收其生殖道，冲洗子宫输卵管连接部（UTJ）以回收精子。与FS输精相比，FT输精后回收的精子较少（p < 0.01），且SP没有明显影响。在实验二中，30头青春期前的后备母猪肌肉注射1000IU eCG，80小时后再注射5mg促黄体素（pLH）以控制排卵时间。在排卵前12小时给后备母猪分别输精3×10⁹个活FS精子（n = 6）、FT精子（n = 15）或FT精子加10% SP（n = 9），8小时后处死。解剖并冲洗UTJ以回收精子。FT输精后回收的精子最少（p < 0.001），且SP没有明显影响。这些数据表明，用FT精子输精的后备母猪的精子库大小明显减小。然而，SP缺乏作用表明，要么它没有逆转低温获能，要么这种逆转不会影响体内形成精子库的能力。
