Hoflack G, Opsomer G, Rijsselaere T, Van Soom A, Maes D, de Kruif A, Duchateau L
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Reprod Domest Anim. 2007 Apr;42(2):153-61. doi: 10.1111/j.1439-0531.2006.00745.x.
Subjective microscopic sperm motility results have recently been demonstrated to differ between Holstein-Friesian (HF) and Belgian Blue (BB) bulls. However, such assessments are rather imprecise. In the present study, sperm motility was assessed objectively by means of the Hamilton Thorne CEROS version 12.2c computer-assisted sperm motility analyser (CASA), and differences between the BB and HF breed could also be demonstrated. Higher percentages of both totally (p < 0.0001) and progressively (p < 0.0001) motile spermatozoa were encountered in the HF breed compared with the BB breed. Furthermore, a lower kinetic efficiency of the BB spermatozoa, evidenced by a lower beat cross-frequency (p = 0.0007) combined with a higher lateral head displacement (p = 0.0015), was the basis for the lower velocity of BB sperm cells. Additionally, BB spermatozoa move less straight forward, resulting in a lower straightness (p < 0.0001). No sperm motility differences were observed between age groups within the BB breed. The breed differences were observed in the examined bull populations residing at AI centres, in Belgium for the BB bulls and in the Netherlands for the HF bulls. However, these bull populations are selected for fertility. A similar pattern was observed in an unselected bull population of both breeds, although these differences were mostly non-significant for the different CASA parameters. Nevertheless, these data suggest that a genetic component might be responsible for the observed sperm motility breed differences.
最近有研究表明，荷斯坦 - 弗里生（HF）公牛和比利时蓝牛（BB）公牛的主观显微镜下精子活力结果存在差异。然而，此类评估相当不精确。在本研究中，通过汉密尔顿·桑恩CEROS 12.2c版计算机辅助精子活力分析仪（CASA）客观评估精子活力，也证实了BB和HF品种之间的差异。与BB品种相比，HF品种中完全（p < 0.0001）和渐进性（p < 0.0001）活动精子的百分比更高。此外，BB精子较低的动力学效率，表现为较低的拍频交叉频率（p = 0.0007）以及较高的头部侧向位移（p = 0.0015），是BB精子细胞速度较低的原因。此外，BB精子向前直线移动较少，导致直线性较低（p < 0.0001）。在BB品种的不同年龄组之间未观察到精子活力差异。在位于人工授精中心的受检公牛群体中观察到了品种差异，BB公牛在比利时，HF公牛在荷兰。然而，这些公牛群体是经过生育力筛选的。在两个品种的未筛选公牛群体中也观察到了类似模式，尽管对于不同的CASA参数，这些差异大多不显著。尽管如此，这些数据表明遗传因素可能是观察到的精子活力品种差异的原因。
