Gross Nicole, Taylor Todd, Crenshaw Thomas, Khatib Hasan
Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI, United States.
Front Genet. 2020 Nov 5;11:597943. doi: 10.3389/fgene.2020.597943. eCollection 2020.
Knowledge of non-genomic inheritance of traits is currently limited. Although it is well established that maternal diet influences offspring inheritance of traits through DNA methylation, studies on the impact of prepubertal paternal diet on DNA methylation are rare. This study aimed to evaluate the impact of prepubertal diet in Polypay rams on complex traits, DNA methylation, and transmission of traits to offspring. A total of 10 littermate pairs of F0 rams were divided so that one ram was fed a control diet, and the other was fed the control diet with supplemental methionine. Diet was associated with earlier age at puberty in treatment vs. control F0 rams. F0 treatment rams tended to show decreased pubertal weight compared to control rams; however, no differences were detected in overall growth. A total of ten F0 rams were bred, and the entire F1 generation was fed a control diet. Diet of F0 rams had a significant association with scrotal circumference (SC) and weight at puberty of F1 offspring. The paternal diet was not significantly associated with F1 ram growth or age at puberty. The DNA methylation of F0 ram sperm was assessed, and genes related to both sexual development (e.g., , , , , , ) and body weight (e.g., , ) were prevalent in the data. These results provide novel information about the mechanisms through which the prepubertal paternal diet may alter body weight at puberty and sexual development.
目前，对性状非基因组遗传的了解有限。尽管已经明确母体饮食通过DNA甲基化影响后代性状的遗传，但关于青春期前父体饮食对DNA甲基化影响的研究却很少。本研究旨在评估青春期前饮食对波利佩公羊复杂性状、DNA甲基化以及性状向后代传递的影响。总共10对同窝出生的F0公羊被分开，使得一只公羊喂食对照饮食，另一只公羊喂食添加蛋氨酸的对照饮食。与对照F0公羊相比，处理组公羊的饮食与青春期年龄提前有关。与对照公羊相比，F0处理组公羊的青春期体重有下降趋势；然而，在总体生长方面未检测到差异。总共10只F0公羊进行配种，整个F1代都喂食对照饮食。F0公羊的饮食与F1后代的阴囊周长（SC）和青春期体重有显著关联。父体饮食与F1公羊的生长或青春期年龄没有显著关联。对F0公羊精子的DNA甲基化进行了评估，数据中普遍存在与性发育（如……）和体重（如……）相关的基因。这些结果提供了关于青春期前父体饮食可能改变青春期体重和性发育机制的新信息。
