Ivanov Boris, Mubarakshina Maria, Khorobrykh Sergey
Institute of Basic Biological Problems, Russian Academy of Sciences, Photosynthetic Electron Transport Lab, Pushchino 142290, Moscow Region, Russian Federation.
FEBS Lett. 2007 Apr 3;581(7):1342-6. doi: 10.1016/j.febslet.2007.02.044. Epub 2007 Mar 1.
The oxidation of the PQ-pool after illumination with 50 or 500 micromol quantam(-2)s(-1) was measured in isolated thylakoids as the increase in DeltaA(263), i.e., as the appearance of PQ. While it was not observed under anaerobic conditions, under aerobic conditions it was biphasic. The first faster phase constituted 26% or 44% of total reappearance of PQ, after weak or strong light respectively. The dependence on oxygen presence as well as the correlation with the rate of oxygen consumption led to conclusion that this phase represents the appearance of PQ from PQ(*-) produced in the course of PQH(2) oxidation by superoxide accumulated in the light within the membrane.
在分离的类囊体中，以ΔA(263)的增加来测量用50或500微摩尔量子（-2）秒（-1）光照后PQ库的氧化，即PQ的出现。虽然在厌氧条件下未观察到，但在需氧条件下它是双相的。第一个较快的阶段分别在弱光或强光后占PQ总再现量的26%或44%。对氧气存在的依赖性以及与耗氧率的相关性得出结论，该阶段代表了膜内光积累的超氧化物在PQH(2)氧化过程中产生的PQ(*-)形成的PQ的出现。
