Takamizawa Satoru, Shibahara Hiroaki, Shibayama Tamaho, Suzuki Mitsuaki
Department of Obstetrics and Gynecology, School of Medicine, Jichi Medical University, Tochigi, Japan.
Fertil Steril. 2007 Oct;88(4):925-32. doi: 10.1016/j.fertnstert.2006.12.029. Epub 2007 Mar 8.
To develop a highly specific test for the detection of antizona pellucida (ZP) antibodies in the sera from premature ovarian failure (POF) patients.
Laboratory study.
University hospital.
PATIENT(S): Twenty-seven idiopathic POF patients, 30 control women, and 30 healthy males.
INTERVENTION(S): Anti-ZP antibodies were detected by the microdot assay using a very small amount of human ZP or porcine ZP. The effect of anti-ZP antibodies on sperm-ZP binding was examined by hemizona assay.
MAIN OUTCOME MEASURE(S): Results from the microdot assay and hemizona assay.
RESULT(S): By the microdot assay using human ZP, the sera from POF patients reacted significantly stronger than those of control women and healthy males. However, no obvious difference could be found by the same assay using porcine ZP among these three groups. Anti-ZP antibodies against sera from some POF patients showed significant blocking effects on sperm-ZP binding assessed by hemizona assay. Anti-ZP antibodies were detected in 7 of 27 POF patients, while none were detected in control women and healthy males.
CONCLUSION(S): Some idiopathic POF patients have anti-ZP antibodies in their sera, which were detected with high specificity by a newly developed microdot assay using a very small amount of human ZP.
开发一种高度特异性的检测方法，用于检测卵巢早衰（POF）患者血清中的抗透明带（ZP）抗体。
实验室研究。
大学医院。
27例特发性POF患者、30名对照女性和30名健康男性。
使用极少量人ZP或猪ZP通过微斑点试验检测抗ZP抗体。通过半透明带试验检测抗ZP抗体对精子-ZP结合的影响。
微斑点试验和半透明带试验的结果。
通过使用人ZP的微斑点试验，POF患者血清的反应明显强于对照女性和健康男性。然而，使用猪ZP进行的相同试验在这三组中未发现明显差异。通过半透明带试验评估，一些POF患者血清中的抗ZP抗体对精子-ZP结合显示出显著的阻断作用。27例POF患者中有7例检测到抗ZP抗体，而对照女性和健康男性中均未检测到。
一些特发性POF患者血清中存在抗ZP抗体，使用极少量人ZP的新开发微斑点试验可高度特异性地检测到这些抗体。
