Liang Xiaorong, McLuckey Scott A
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.
J Am Soc Mass Spectrom. 2007 May;18(5):882-90. doi: 10.1016/j.jasms.2007.02.001. Epub 2007 Mar 8.
A new method is described for effecting ion/ion proton transfer reactions that involves storage of analyte ions while oppositely charged ions are transmitted through the stored ion population. In this approach, the products are captured and stored in the linear ion trap for subsequent mass analysis. Charge reduction of multiply charged protein ions is used as an example to illustrate the analytical usefulness of this method. In another variation of the transmission mode ion/ion reaction approach, two charge inversion experiments, implemented by passing analyte ions through a population of multiply charged reagent ions in a LIT, are also demonstrated. A pulsed dual ion source approach coupled with a hybrid triple quadrupole/linear ion trap instrument was used to demonstrate these two methods. The results for ion/ion reactions implemented using these so-called "transmission mode" experiments were comparable to those acquired using the more conventional mutual storage mode, both in terms of efficiency and information content of the spectra. An advantage of transmission mode experiments compared with mutual storage mode experiments is that they do not require any specialized measures to be taken to enable the simultaneous storage of oppositely charged ions.
本文描述了一种实现离子/离子质子转移反应的新方法，该方法涉及在带相反电荷的离子穿过存储的离子群体时存储分析物离子。在这种方法中，产物被捕获并存储在线性离子阱中，以便后续进行质量分析。以多电荷蛋白质离子的电荷减少为例，说明该方法的分析实用性。在传输模式离子/离子反应方法的另一种变体中，还展示了通过使分析物离子穿过线性离子阱中的多电荷试剂离子群体来实施的两个电荷反转实验。使用脉冲双离子源方法与混合三重四极杆/线性离子阱仪器相结合来演示这两种方法。使用这些所谓的“传输模式”实验进行离子/离子反应的结果，在光谱的效率和信息含量方面，与使用更传统的相互存储模式获得的结果相当。与相互存储模式实验相比，传输模式实验的一个优点是它们不需要采取任何专门措施来实现带相反电荷离子的同时存储。
