Chen Meixue, Yan Lizhu, He Hong, Chang Qingyun, Yu Yunbo, Qu Jiuhui
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
J Inorg Biochem. 2007 May;101(5):817-23. doi: 10.1016/j.jinorgbio.2007.01.008. Epub 2007 Feb 3.
Bactericidal action of Al(2)O(3), Ag/Al(2)O(3) and AgCl/Al(2)O(3) on pure culture of Escherichia coli K 12 was studied. Ag/Al(2)O(3) and AgCl/Al(2)O(3) demonstrated a stronger bactericidal activity than Al(2)O(3). The colony-forming ability of E. coli was completely lost in 0.5 min on both of Ag/Al(2)O(3) and AgCl/Al(2)O(3) at room temperature in air. The configuration of the bacteria on the catalyst surface was observed using scanning electron microscopy (SEM). Reactive oxygen species (ROS) play an important role in the expression of the bactericidal activity on the surface of catalysts by assay with O(2)/N(2) bubbling and scavenger for ROS. Furthermore, the formation of CO(2) as an oxidation product could be detected by diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and be deduced by total carbon analysis. These results strongly support that the bactericidal process on the surface of Ag/Al(2)O(3) and AgCl/Al(2)O(3) was caused by the catalytic oxidation.
研究了Al₂O₃、Ag/Al₂O₃和AgCl/Al₂O₃对大肠杆菌K12纯培养物的杀菌作用。Ag/Al₂O₃和AgCl/Al₂O₃表现出比Al₂O₃更强的杀菌活性。在室温空气中，大肠杆菌在Ag/Al₂O₃和AgCl/Al₂O₃上0.5分钟内菌落形成能力就完全丧失。使用扫描电子显微镜（SEM）观察了催化剂表面细菌的形态。通过O₂/N₂鼓泡和活性氧清除剂的测定，活性氧（ROS）在催化剂表面杀菌活性的表达中起重要作用。此外，作为氧化产物的CO₂的形成可以通过漫反射红外傅里叶变换光谱（DRIFTS）检测到，并通过总碳分析推断出来。这些结果有力地支持了Ag/Al₂O₃和AgCl/Al₂O₃表面的杀菌过程是由催化氧化引起的。
