Research Institute for Basic Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513, Republic of Korea; Department of Microbiology, College of Natural Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513, Republic of Korea.
Department of Microbiology, College of Natural Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513, Republic of Korea; Institute of Marine Biotechnology, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513, Republic of Korea.
Microb Pathog. 2018 Mar;116:84-90. doi: 10.1016/j.micpath.2018.01.018. Epub 2018 Jan 12.
Facile, eco-friendly synthesis of metal nanoparticles has been proposed as a cost effective method. In the present study, we propose the facile synthesis of silver-silver chloride (Ag-AgCl) nanoparticles (NPs) using the medicinally important Agrimonia pilosa plant extract without addition of capping or stabilizing agents. The Ag-AgCl NPs synthesis was observed at 40 °C after 10 min incubation; the synthesis of Ag-AgCl NPs was indicated by color change and confirmed by UV-vis spectroscopic peak at 454 nm. TEM analysis confirmed Ag-AgCl NPs were 10-20 nm in size and spherical, and oval in shape. Elemental composition was determined by energy dispersive X-ray analysis, and crystalline structure was confirmed by X-ray diffraction spectroscopy. Different phytocomponents present in the plant extract were analyzed by Gas Chromatography-Mass spectrometry, and the interaction of biomolecules in reduction process was analyzed by Fourier transform infrared spectroscopy studies. The synthesized Ag-AgCl NPs showed significant antibacterial efficiency, analyzed by well diffusion assay against pathogenic bacteria including Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus saprophyticus, Escherichia coli, Pseudomonas putida. Minimum inhibitory concentration and minimum bactericidal concentration were evaluated by microbroth dilution, and spread plate method, respectively. The possible mechanism of bacterial growth inhibition is due to changes in bacterial cell wall morphology that was studied by FE-SEM analysis.
已经提出了一种简便、环保的金属纳米粒子合成方法，作为一种具有成本效益的方法。在本研究中，我们提出了一种简便的方法，使用具有药用重要性的龙牙草植物提取物合成银-氯化银（Ag-AgCl）纳米粒子（NPs），无需添加封端或稳定剂。Ag-AgCl NPs 的合成在 40°C 下孵育 10 分钟后观察到；Ag-AgCl NPs 的合成通过颜色变化和在 454nm 处的紫外可见光谱峰来证实。TEM 分析证实 Ag-AgCl NPs 的尺寸为 10-20nm，呈球形和椭圆形。通过能量色散 X 射线分析确定元素组成，并通过 X 射线衍射光谱学确认晶体结构。通过气相色谱-质谱分析鉴定了植物提取物中存在的不同植物成分，并通过傅里叶变换红外光谱研究分析了生物分子在还原过程中的相互作用。通过琼脂扩散法对包括蜡样芽孢杆菌、单核细胞增生李斯特菌、金黄色葡萄球菌、腐生葡萄球菌、大肠杆菌、铜绿假单胞菌在内的病原菌进行了抗菌效率分析，结果表明所合成的 Ag-AgCl NPs 具有显著的抗菌效率。通过微量肉汤稀释法和涂布平板法分别评估最小抑菌浓度和最小杀菌浓度。通过 FE-SEM 分析研究了细菌生长抑制的可能机制，这是由于细菌细胞壁形态的变化所致。
