Marsh Susan A, Powell Pamela C, Agarwal Anupam, Dell'Italia Louis J, Chatham John C
Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham, AL 35294-0005, USA.
Am J Physiol Heart Circ Physiol. 2007 Jul;293(1):H292-8. doi: 10.1152/ajpheart.01362.2006. Epub 2007 Mar 9.
Recent studies in our laboratory using the Zucker obese (ZO) and Zucker diabetic fatty (ZDF) rat models resulted in unexpectedly high mortality rates in all genotypes including healthy homozygous lean Zucker rats, possibly because of renal dysfunction. Therefore, we evaluated left ventricular (LV) and kidney morphology and function in young ZO, Zucker diabetic fatty obese (ZDFO), homozygous Zucker/ZDF lean (ZL), and Sprague-Dawley (SD) rats. Hydronephrosis was evident in ZL, ZO, and ZDFO but not SD kidneys. ZDFO rats exhibited impaired LV shortening and relaxation with increased arterial stiffness. LV wall thickness was lower and LV end-systolic wall stress was higher in ZDFO compared with SD rats. Plasma ANG II was lower in ZO and ZDFO rats, which may be a result of reduced renal parenchyma with hydronephrosis; norepinephrine was higher in ZDFO rats than SD controls. Covariate analysis indicated that LV end-systolic wall stress was associated with renal dysfunction. The presence of hydronephrosis and its association with LV dysfunction potentially limits the ZDF model for study of the effects of diabetes on renal and cardiovascular function.
我们实验室最近使用祖克肥胖（ZO）和祖克糖尿病脂肪（ZDF）大鼠模型进行的研究发现，包括健康的纯合瘦型祖克大鼠在内的所有基因型的死亡率都意外地高，这可能是由于肾功能不全所致。因此，我们评估了年轻的ZO、祖克糖尿病脂肪肥胖（ZDFO）、纯合祖克/ZDF瘦型（ZL）和斯普拉格-道利（SD）大鼠的左心室（LV）以及肾脏的形态和功能。ZL、ZO和ZDFO大鼠的肾脏出现肾盂积水，而SD大鼠则未出现。ZDFO大鼠表现出左心室缩短和舒张功能受损，动脉僵硬度增加。与SD大鼠相比，ZDFO大鼠的左心室壁厚度更低，左心室收缩末期壁应力更高。ZO和ZDFO大鼠的血浆血管紧张素II水平较低，这可能是肾盂积水导致肾实质减少的结果；ZDFO大鼠的去甲肾上腺素水平高于SD对照组。协变量分析表明，左心室收缩末期壁应力与肾功能不全有关。肾盂积水的存在及其与左心室功能障碍的关联可能限制了ZDF模型在研究糖尿病对肾脏和心血管功能影响方面的应用。
