Hiscott P, Waller H A, Grierson I, Butler M G, Scott D
Department of Pathology, Institute of Ophthalmology, London, UK.
Cell Tissue Res. 1992 Jan;267(1):185-92. doi: 10.1007/BF00318703.
The distribution of fibronectin mRNA and fibronectin in adult human retina and epiretinal membranes was investigated by in situ hybridisation and immunohistochemical techniques. The cells in normal adult retina contained little or no fibronectin mRNA and the retina only showed fibronectin immunoreactivity in retinal vessels. The cells in detached neuroretina did not contain fibronectin message but the vitreoretinal interface of the detached retina exhibited variable fibronectin immunoreactivity. Retinal glia, retinal pigment epithelium and fibroblast-like cells in membranes at the vitreoretinal juncture (epiretinal membranes) showed variable labelling with the fibronectin mRNA probe and all the membranes immunostained for fibronectin. No difference could be detected between membrane cell types in the intensity of labelling with the mRNA probe or for fibronectin immunoreactivity. The results indicate that cells in situ in attached and detached adult human retina do not produce fibronectin. Although fibronectin at the vitreoretinal juncture in retinal detachment is probably partly derived from plasma fibronectin resulting from breakdown of the blood-retinal barrier, ectopic retinal cells produce fibronectin and contribute to the glycoprotein epiretinal membranes.
通过原位杂交和免疫组织化学技术研究了纤连蛋白mRNA和纤连蛋白在成人视网膜和视网膜前膜中的分布。正常成人视网膜中的细胞几乎不含有或不含有纤连蛋白mRNA，视网膜仅在视网膜血管中显示纤连蛋白免疫反应性。脱离的神经视网膜中的细胞不含有纤连蛋白信息，但脱离视网膜的玻璃体视网膜界面表现出可变的纤连蛋白免疫反应性。视网膜胶质细胞、视网膜色素上皮细胞和玻璃体视网膜交界处（视网膜前膜）膜中的成纤维细胞样细胞用纤连蛋白mRNA探针显示出可变的标记，并且所有膜都用纤连蛋白进行免疫染色。在用mRNA探针标记的强度或纤连蛋白免疫反应性方面，膜细胞类型之间未检测到差异。结果表明，附着和脱离的成人视网膜中的原位细胞不产生纤连蛋白。尽管视网膜脱离时玻璃体视网膜交界处的纤连蛋白可能部分源自血视网膜屏障破坏产生的血浆纤连蛋白，但异位视网膜细胞产生纤连蛋白并有助于形成糖蛋白视网膜前膜。
