Hiscott P S, Grierson I, McLeod D
Br J Ophthalmol. 1984 Oct;68(10):708-15. doi: 10.1136/bjo.68.10.708.
Immunohistochemical techniques were used to identify cells containing cytokeratins in sections or tissue-culture monolayers from ocular (reference) tissues and also from 22 epiretinal membranes obtained during closed microsurgery for macular pucker or massive preretinal retraction. Results of cytokeratin immunostaining in reference tissues indicated that this is a valuable means of determining the contribution and distribution of epithelial cells in epiretinal membranes, and that the epithelial cells in the membranes were probably derived from the retinal pigment epithelium. Epithelial cells were identified in 17 of the 22 epiretinal membranes, but they did not usually constitute the predominant cell type. We concluded that the fibroblasts or fibroblast-like cells thought to be responsible for the contraction of epiretinal membranes are seldom of retinal pigment epithelial origin. Biomicroscopic pigmentation of a membrane was shown to be a poor guide to its epithelial cell population.
免疫组织化学技术用于鉴定来自眼部（对照）组织以及在黄斑皱襞或大量视网膜前膜脱离的闭合显微手术中获取的22个视网膜前膜的切片或组织培养单层中含有细胞角蛋白的细胞。对照组织中细胞角蛋白免疫染色的结果表明，这是确定视网膜前膜中上皮细胞的贡献和分布的一种有价值的方法，并且膜中的上皮细胞可能来源于视网膜色素上皮。在22个视网膜前膜中的17个中鉴定出了上皮细胞，但它们通常不构成主要的细胞类型。我们得出结论，被认为负责视网膜前膜收缩的成纤维细胞或成纤维细胞样细胞很少起源于视网膜色素上皮。膜的生物显微镜下色素沉着显示对其上皮细胞群体的指导作用较差。
