Gao Shang-pu, Huang Lan, Yang Xin-wei
Department of Dermatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Zhong Xi Yi Jie He Xue Bao. 2007 Mar;5(2):170-3. doi: 10.3736/jcim20070214.
To investigate the possible stimulating mechanism of Huoxue Bushen Mixture (HXBSM), a traditional Chinese compound medicine, on hair growth of mice via measuring the variance of skin blood vessel neogenesis and vascular endothelial growth factor (VEGF) expression in the hair follicle.
Hot rosin and paraffin mixture depilation were used to induce C57BL/6 mice hair follicle to enter from telogen into anagen. Ninety C57BL/6 mice were divided into 3 groups randomly: HXBSM group, Yangxue Shengfa Capsule (YXSFC, another traditional Chinese compound medicine) group and untreated group. The mice were fed with corresponding drugs after modeling. The hair growth of the mice was observed every day. Every ten mice out of each group were executed respectively at day 4, 11 and day 17. Skin blood vessel neogenesis was counted through pathological section and VEGF expression in the hair follicle was measured via immunohistochemical method.
The number of local blood vessel neogenisis in the HXBSM group observed was larger than that in the untreated group at day 4 (P<0.05); and evidently larger than that in the YXSFC group and the untreated group at day 11 (P<0.05). The expression of VEGF in the hair follicle was distinctively higher than that in the YXSFC group and the untreated group at day 11 and day 17 (P<0.05).
HXBSM up-regulates VEGF expression to accelerate blood vessel neogenesis and hair growth.
通过检测毛囊皮肤血管新生及血管内皮生长因子（VEGF）表达的变化，探讨中药复方活血补肾合剂（HXBSM）对小鼠毛发生长的可能刺激机制。
采用热松香和石蜡混合物脱毛法诱导C57BL/6小鼠毛囊从休止期进入生长期。将90只C57BL/6小鼠随机分为3组：HXBSM组、养血生发胶囊（YXSFC，另一种中药复方）组和未处理组。造模后给小鼠喂食相应药物。每天观察小鼠的毛发生长情况。每组分别在第4天、第11天和第17天处死10只小鼠。通过病理切片计数皮肤血管新生情况，采用免疫组化法检测毛囊中VEGF的表达。
第4天，HXBSM组观察到的局部血管新生数量高于未处理组（P<0.05）；第11天，明显高于YXSFC组和未处理组（P<0.05）。第11天和第17天，毛囊中VEGF的表达明显高于YXSFC组和未处理组（P<0.05）。
HXBSM上调VEGF表达，加速血管新生和毛发生长。
