Valentine Elizabeth R, Ferrage Fabien, Massi Francesca, Cowburn David, Palmer Arthur G
Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
J Biomol NMR. 2007 May;38(1):11-22. doi: 10.1007/s10858-006-9131-9. Epub 2007 Mar 13.
Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346-5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711-6721; Kupce E, Freeman R (1997) J Magn Reson 127:36-48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond (1)H-(13)C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from (1)H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with (13)C and (15)N isotopes.
联合复合旋转绝热扫描同位素滤波器是通过结合复合旋转[斯图尔特·A·C等人（1999年）《美国化学会志》121: 5346 - 5347]和绝热扫描[茨瓦伦·C等人（1997年）《美国化学会志》119: 6711 - 6721；库普采·E，弗里曼·R（1997年）《磁共振杂志》127: 36 - 48]方法推导出来的。联合同位素滤波器具有改进的宽带过滤性能，即使对于蛋白质和RNA分子中一键（1）H -（13）C标量耦合常数的极值情况也是如此。采用平均哈密顿量分析来描述同位素滤波器序列内绝热扫描期间异核标量耦合相互作用的演化。新的同位素滤波器元件能够改进对源自与复合物未标记天然丰度组分相连的（1）H自旋的NMR共振信号的选择性检测，该复合物的其他组分用（13）C和（15）N同位素标记。
