在摇瓶和生物反应器中培养的古古勒树（Commiphora wightii）细胞悬浮培养物中古古甾酮的生产。
Guggulsterone production in cell suspension cultures of the guggul tree, Commiphora wightii, grown in shake-flasks and bioreactors.
作者信息
Mathur Meeta, Ramawat K G
机构信息
Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University, Udaipur, India.
出版信息
Biotechnol Lett. 2007 Jun;29(6):979-82. doi: 10.1007/s10529-007-9342-5. Epub 2007 Mar 13.
PMID:17354018
Abstract
Cell suspension cultures of Commiphora wightii, grown in modified MS medium containing 2,4-dichlorophenoxyacetic acid (0.5 mg l(-1)) and kinetin (0.25 mg l(-1)), produced approximately 5 microg guggulsterone g(-1) dry wt. In a 2 l stirred tank bioreactor, the biomass was 5.5 g l(-1) and total guggulsterone was 36 microg l(-1).
摘要
在含有2,4 - 二氯苯氧乙酸（0.5 mg l(-1)）和激动素（0.25 mg l(-1)）的改良MS培养基中生长的印度没药细胞悬浮培养物，每克干重产生约5微克的古甾酮。在一个2升的搅拌罐式生物反应器中，生物量为5.5 g l(-1)，总古甾酮含量为36 microg l(-1)。
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