Kulhari Alpana, Sheorayan Arun, Chaudhury Ashok, Sarkar Susheel, Kalia Rajwant K
Center for Plant Biotechnology, CCSHAU New Campus, Hisar, India ; Department of Bio and Nanotechnology, Guru Jambeshwar University of Science and Technology, Hisar, India.
Department of Bio and Nanotechnology, Guru Jambeshwar University of Science and Technology, Hisar, India.
Physiol Mol Biol Plants. 2015 Jan;21(1):71-81. doi: 10.1007/s12298-014-0271-1. Epub 2014 Nov 22.
Guggulsterone is an aromatic steroidal ketonic compound obtained from vertical rein ducts and canals of bark of Commiphora wightii (Arn.) Bhandari (Family - Burseraceae). Owing to its multifarious medicinal and therapeutic values as well as its various other significant bioactivities, guggulsterone has high demand in pharmaceutical, perfumery and incense industries. More and more pharmaceutical and perfumery industries are showing interest in guggulsterone, therefore, there is a need for its quantitative determination in existing natural populations of C. wightii. Identification of elite germplasm having higher guggulsterone content can be multiplied through conventional or biotechnological means. In the present study an effort was made to estimate two isoforms of guggulsterone i.e. E and Z guggulsterone in raw exudates of 75 accessions of C. wightii collected from three states of North-western India viz. Rajasthan (19 districts), Haryana (4 districts) and Gujarat (3 districts). Extracted steroid rich fraction from stem samples was fractionated using reverse-phase preparative High Performance Liquid Chromatography (HPLC) coupled with UV/VIS detector operating at wavelength of 250 nm. HPLC analysis of stem samples of wild as well as cultivated plants showed that the concentration of E and Z isomers as well as total guggulsterone was highest in Rajasthan, as compared to Haryana and Gujarat states. Highest concentration of E guggulsterone (487.45 μg/g) and Z guggulsterone (487.68 μg/g) was found in samples collected from Devikot (Jaisalmer) and Palana (Bikaner) respectively, the two hyper-arid regions of Rajasthan, India. Quantitative assay was presented on the basis of calibration curve obtained from a mixture of standard E and Z guggulsterones with different validatory parameters including linearity, selectivity and specificity, accuracy, auto-injector, flow-rate, recoveries, limit of detection and limit of quantification (as per norms of International conference of Hormonization). Present findings revealed the role of environmental factors on biosynthesis of guggulsterone isomers under natural conditions.
古古甾酮是一种芳香甾体酮类化合物，从印度没药（Commiphora wightii (Arn.) Bhandari，橄榄科）树皮的垂直树脂道和管道中提取。由于其具有多种药用和治疗价值以及其他各种重要的生物活性，古古甾酮在制药、香料和香薰行业有很高的需求。越来越多的制药和香料行业对古古甾酮表现出兴趣，因此，需要对印度没药现有的自然种群中的古古甾酮进行定量测定。可以通过传统或生物技术手段繁殖具有较高古古甾酮含量的优良种质。在本研究中，我们对从印度西北部三个邦（拉贾斯坦邦（19个区）、哈里亚纳邦（4个区）和古吉拉特邦（3个区））收集的75份印度没药种质的生渗出物中的两种古古甾酮异构体（即E型和Z型古古甾酮）进行了估算。从茎样本中提取的富含类固醇的部分使用反相制备高效液相色谱（HPLC）进行分离，并结合在250 nm波长下运行的紫外/可见检测器。对野生和栽培植物的茎样本进行的HPLC分析表明，与哈里亚纳邦和古吉拉特邦相比，拉贾斯坦邦的E型和Z型异构体以及总古古甾酮的浓度最高。在分别从印度拉贾斯坦邦的两个超干旱地区德维科特（斋沙默尔）和帕拉纳（比卡内尔）采集的样本中，发现E型古古甾酮（487.45 μg/g）和Z型古古甾酮（487.68 μg/g）的浓度最高。基于从标准E型和Z型古古甾酮混合物获得的校准曲线进行了定量分析，并给出了包括线性、选择性和特异性、准确性、自动进样器、流速、回收率、检测限和定量限（按照国际协调会议的规范）等不同验证参数。目前的研究结果揭示了环境因素在自然条件下对古古甾酮异构体生物合成的作用。
